PMID- 24626965
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20211021
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 9
IP  - 5
DP  - 2014 May
TI  - Anti-inflammatory and anti-proliferative effect of herbal medicines (APR) in 
      RAW264.7 cells.
PG  - 1569-74
LID - 10.3892/mmr.2014.2033 [doi]
AB  - The objective of the present study was to analyze the effect of a mixture of 
      medicinal plants [Angelica gigas Nakai, Panax ginseng and Rhus verniciflua Stokes 
      (APR)] on lipopolysaccharide (LPS)-induced inflammatory responses in the murine 
      macrophage cell line RAW264.7. Cells were treated with APR and LPS at various 
      concentrations and indicated times. WST assay, trypan blue assay and 
      quantification of activated cells demonstrated that APR suppressed cell 
      proliferation in a dose-dependent manner. APR induced G1 cell cycle arrest and 
      inhibited the LPS-induced phosphorylation of protein kinase B (AKT), 
      extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase 
      (p38) and necrosis factor kappaB (NF-kappaB). APR also suppressed nitric oxide synthase 
      isoform (iNOS) and prostaglandin endoperoxide synthase 2 (Cox-2) messenger 
      ribonucleic acid (mRNA) expression induced by LPS. Furthermore, APR decreased 
      LPS-induced intracellular reactive oxygen species (ROS) levels, mitochondrial 
      membrane potential, as well as induced PARP and caspase-3 cleavage, suggesting 
      that APR causes apoptosis. In conclusion, the present study indicated that APR 
      may be advantageous in treating inflammatory disease.
FAU - Choi, Han-Seok
AU  - Choi HS
AD  - Laboratory of Clinical Biology and Pharmacogenomics and Center for Clinical 
      Research and Genomics, Institute of Oriental Medicine, Kyung Hee University, 
      Seoul 130-701, Republic of Korea.
FAU - Seo, Hye Sook
AU  - Seo HS
AD  - Laboratory of Clinical Biology and Pharmacogenomics and Center for Clinical 
      Research and Genomics, Institute of Oriental Medicine, Kyung Hee University, 
      Seoul 130-701, Republic of Korea.
FAU - Kim, Soon Re
AU  - Kim SR
AD  - Laboratory of Clinical Biology and Pharmacogenomics and Center for Clinical 
      Research and Genomics, Institute of Oriental Medicine, Kyung Hee University, 
      Seoul 130-701, Republic of Korea.
FAU - Choi, Youn Kyung
AU  - Choi YK
AD  - Laboratory of Clinical Biology and Pharmacogenomics and Center for Clinical 
      Research and Genomics, Institute of Oriental Medicine, Kyung Hee University, 
      Seoul 130-701, Republic of Korea.
FAU - Shin, Yong-Cheol
AU  - Shin YC
AD  - Laboratory of Clinical Biology and Pharmacogenomics and Center for Clinical 
      Research and Genomics, Institute of Oriental Medicine, Kyung Hee University, 
      Seoul 130-701, Republic of Korea.
FAU - Ko, Seong-Gyu
AU  - Ko SG
AD  - Laboratory of Clinical Biology and Pharmacogenomics and Center for Clinical 
      Research and Genomics, Institute of Oriental Medicine, Kyung Hee University, 
      Seoul 130-701, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140310
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Cycle/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors
MH  - Gene Expression Regulation/drug effects
MH  - *Herbal Medicine
MH  - Macrophages/*drug effects/metabolism
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mice
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - Plant Extracts/pharmacology
MH  - Plants, Medicinal/chemistry
MH  - Proto-Oncogene Proteins c-akt/antagonists & inhibitors
MH  - Reactive Oxygen Species/metabolism
PMC - PMC4020494
EDAT- 2014/03/15 06:00
MHDA- 2014/11/19 06:00
PMCR- 2014/03/10
CRDT- 2014/03/15 06:00
PHST- 2013/08/13 00:00 [received]
PHST- 2014/02/24 00:00 [accepted]
PHST- 2014/03/15 06:00 [entrez]
PHST- 2014/03/15 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
PHST- 2014/03/10 00:00 [pmc-release]
AID - mmr-09-05-1569 [pii]
AID - 10.3892/mmr.2014.2033 [doi]
PST - ppublish
SO  - Mol Med Rep. 2014 May;9(5):1569-74. doi: 10.3892/mmr.2014.2033. Epub 2014 Mar 10.