PMID- 24627693
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140314
LR  - 20220408
IS  - 1738-1843 (Print)
IS  - 2092-8920 (Electronic)
IS  - 1738-1843 (Linking)
VI  - 48
IP  - 1
DP  - 2014 Feb
TI  - Effects of fixation and storage of human tissue samples on nucleic Acid 
      preservation.
PG  - 36-42
LID - 10.4132/KoreanJPathol.2014.48.1.36 [doi]
AB  - BACKGROUND: Because of recent advances in the molecular diagnosis of cancer 
      patients, tissue quality has become more important in daily practice. METHODS: To 
      evaluate the effects of fixative, duration of fixation, decalcification, and 
      storage periods on nucleic acid integrity, DNA and RNA were extracted from 
      gastrointestinal cancer tissue. The yield and purity were analyzed, and 
      polymerase chain reaction (PCR) for glyceraldehyde 3-phosphate dehydrogenase 
      (GAPDH; 60 bp), beta-actin (148 bp), and human growth hormone (hGH; 434 bp) and 
      real-time reverse transcription-PCR for beta-actin (97 bp) were performed. RESULTS: 
      All formalin-fixed paraffin-embedded (FFPE) and methacarn-fixed paraffin-embedded 
      (MFPE) samples tested positive for GAPDH and beta-actin by PCR. hGH was successfully 
      detected in all MFPE samples, but in only 46.7% of the FFPE samples. Prolonged 
      formalin fixation resulted in fewer GAPDH and beta-actin PCR products, and 
      amplification of hGH was not successful. The PCR and reverse transcription-PCR 
      results were significantly affected by the duration of decalcification. The 
      yield, purity, and integrity of mRNA progressively decreased with increased 
      storage periods of paraffin blocks. CONCLUSIONS: Fixation and storage should 
      therefore be standardized in order to improve the quality of molecular pathologic 
      diagnosis.
FAU - Nam, Soo Kyung
AU  - Nam SK
AD  - Department of Pathology, Seoul National University Bundang Hospital, Seoul 
      National University College of Medicine, Seongnam, Korea.
FAU - Im, Joon
AU  - Im J
AD  - Department of Pathology, Seoul National University Bundang Hospital, Seoul 
      National University College of Medicine, Seongnam, Korea.
FAU - Kwak, Yoonjin
AU  - Kwak Y
AD  - Department of Pathology, Seoul National University College of Medicine, Seoul, 
      Korea.
FAU - Han, Nayoung
AU  - Han N
AD  - Department of Pathology, Seoul National University College of Medicine, Seoul, 
      Korea.
FAU - Nam, Kyung Han
AU  - Nam KH
AD  - Department of Pathology, Seoul National University Bundang Hospital, Seoul 
      National University College of Medicine, Seongnam, Korea.
FAU - Seo, An Na
AU  - Seo AN
AD  - Department of Pathology, Seoul National University Bundang Hospital, Seoul 
      National University College of Medicine, Seongnam, Korea.
FAU - Lee, Hye Seung
AU  - Lee HS
AD  - Department of Pathology, Seoul National University Bundang Hospital, Seoul 
      National University College of Medicine, Seongnam, Korea.
LA  - eng
PT  - Journal Article
DEP - 20140225
PL  - Korea (South)
TA  - Korean J Pathol
JT  - Korean journal of pathology
JID - 101275976
PMC - PMC3950233
OTO - NOTNLM
OT  - Fixation procedures
OT  - Nucleic acid preservation
OT  - Storage time
COIS- No potential conflict of interest relevant to this article was reported.
EDAT- 2014/03/15 06:00
MHDA- 2014/03/15 06:01
PMCR- 2014/02/01
CRDT- 2014/03/15 06:00
PHST- 2013/11/04 00:00 [received]
PHST- 2014/01/22 00:00 [revised]
PHST- 2014/01/27 00:00 [accepted]
PHST- 2014/03/15 06:00 [entrez]
PHST- 2014/03/15 06:00 [pubmed]
PHST- 2014/03/15 06:01 [medline]
PHST- 2014/02/01 00:00 [pmc-release]
AID - 10.4132/KoreanJPathol.2014.48.1.36 [doi]
PST - ppublish
SO  - Korean J Pathol. 2014 Feb;48(1):36-42. doi: 10.4132/KoreanJPathol.2014.48.1.36. 
      Epub 2014 Feb 25.