PMID- 2462885
OWN - NLM
STAT- MEDLINE
DCOM- 19890216
LR  - 20151119
IS  - 0735-1631 (Print)
IS  - 0735-1631 (Linking)
VI  - 6
IP  - 1
DP  - 1989 Jan
TI  - Diagnosis of intra-amniotic infection: the acridine orange stain.
PG  - 41-5
AB  - The rapid diagnosis of intra-amniotic infection in patients with premature 
      rupture of the membranes (PROM) and preterm labor is of utmost importance. The 
      Gram's stain examination of amniotic fluid can only detect half of these 
      infections. The acridine orange (AO) stain has been claimed to be superior to the 
      Gram's stain in the detection of bacteria in biologic fluids. Therefore, we 
      undertook the present study to examine the value of AO in the detection of 
      bacteria in amniotic fluid. One hundred and thirty-eight patients with a 
      culture-documented prevalence of intra-amniotic infection of 23.2% were studied. 
      The diagnostic indices of the AO were: sensitivity, 43.8%, specificity, 97.2%, 
      positive predictive value, 82.8%, and negative predictive value, 85.1%; the 
      diagnostic indices for the Gram's stain were: sensitivity, 46.8%, specificity, 
      98.1%, positive predictive value, 88.2%, and negative predictive value, 85.9%. 
      There was no difference in the diagnostic values of the tests. The agreement 
      between the two techniques was substantial, as indicated by a kappa index of 0.72 
      (SE = 0.17, p less than 0.001). The AO offered no significant advantage over the 
      Gram's stain examination of amniotic fluid in the detection of intra-amniotic 
      infection. However, the AO stain was able to identify mycoplasma infections that 
      escaped detection by the Gram's stain. Therefore, the AO is presently the only 
      microscopic technique capable of detecting mycoplasma in amniotic fluid.
FAU - Romero, R
AU  - Romero R
AD  - Department of Obstetrics and Gynecology, Yale University School of Medicine, New 
      Haven, Connecticut 06510.
FAU - Emamian, M
AU  - Emamian M
FAU - Quintero, R
AU  - Quintero R
FAU - Wan, M
AU  - Wan M
FAU - Scioscia, A L
AU  - Scioscia AL
FAU - Hobbins, J C
AU  - Hobbins JC
FAU - Edberg, S
AU  - Edberg S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Perinatol
JT  - American journal of perinatology
JID - 8405212
RN  - 0 (Gram's stain)
RN  - 0 (Phenazines)
RN  - F30N4O6XVV (Acridine Orange)
RN  - J4Z741D6O5 (Gentian Violet)
SB  - IM
MH  - *Acridine Orange
MH  - Amniotic Fluid/microbiology
MH  - Bacterial Infections/*diagnosis/etiology
MH  - Chorioamnionitis/*diagnosis/etiology
MH  - Female
MH  - Fetal Membranes, Premature Rupture/complications
MH  - Gentian Violet
MH  - Humans
MH  - Obstetric Labor, Premature/complications
MH  - Phenazines
MH  - Pregnancy
MH  - Staining and Labeling
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1055/s-2007-999542 [doi]
PST - ppublish
SO  - Am J Perinatol. 1989 Jan;6(1):41-5. doi: 10.1055/s-2007-999542.