PMID- 24630970
OWN - NLM
STAT- MEDLINE
DCOM- 20140623
LR  - 20140421
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 542
IP  - 2
DP  - 2014 Jun 1
TI  - Core promoter regulates the expression of cathepsin B gene in the fat body of 
      Bombyx mori.
PG  - 232-9
LID - S0378-1119(14)00290-X [pii]
LID - 10.1016/j.gene.2014.03.012 [doi]
AB  - Bombyx mori cathepsin B (BmCatB) is involved in the programmed cell death of the 
      fat body during B. mori metamorphosis. For a better understanding of the 
      functional regulatory mechanism, the promoter region of BmCatB in the 
      transcriptional regulation has been identified and analyzed in the present study. 
      BmCatB promoter region performed by the 5' truncation or mutagenesis of EcREs was 
      inserted in the pFA3Luc-A3RL double fluorescence expression vector to activate 
      the fireflies luciferase (FLuc) gene. The results indicated that the 
      dual-luciferase activity of BmCatB gene in the silkworm larval fat body is 
      regulated by the length of promoter. Site-directed mutagenesis of EcRE experiment 
      has shown that the EcREs are up-regulated significantly in the regulation of the 
      BmCatB promoter. A 142bp region (-1165 to -1023) and EcREs are the mainly 
      fat-body tissue-specificity related region and could function as a core promoter 
      element.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Cai, Xiang-Yun
AU  - Cai XY
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China.
FAU - Yu, Jie
AU  - Yu J
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China.
FAU - Yu, Hai-Yan
AU  - Yu HY
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China.
FAU - Liu, Yan-Wei
AU  - Liu YW
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China.
FAU - Fang, Yin
AU  - Fang Y
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China.
FAU - Ren, Zi-Xu
AU  - Ren ZX
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China.
FAU - Jia, Jun-Qiang
AU  - Jia JQ
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China; 
      Sericultural Research Institute, Chinese Academy of Agricultural Sciences, 
      Zhenjiang 212018, China.
FAU - Zhang, Guo-Zheng
AU  - Zhang GZ
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China; 
      Sericultural Research Institute, Chinese Academy of Agricultural Sciences, 
      Zhenjiang 212018, China.
FAU - Guo, Xi-Jie
AU  - Guo XJ
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China; 
      Sericultural Research Institute, Chinese Academy of Agricultural Sciences, 
      Zhenjiang 212018, China.
FAU - Jin, Byung-Rae
AU  - Jin BR
AD  - College of Natural Resources and Life Science, Dong-A University, Busan 604-714, 
      Republic of Korea.
FAU - Gui, Zhong-Zheng
AU  - Gui ZZ
AD  - Jiangsu University of Science and Technology, Zhenjiang 212018, China; 
      Sericultural Research Institute, Chinese Academy of Agricultural Sciences, 
      Zhenjiang 212018, China. Electronic address: srizzgui@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140311
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Insect Proteins)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 3.4.22.1 (Cathepsin B)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Base Sequence
MH  - Bombyx/*genetics/physiology
MH  - Cathepsin B/*genetics/metabolism
MH  - Fat Body/*physiology
MH  - Gene Expression Regulation
MH  - Insect Proteins/*genetics/metabolism
MH  - Luciferases/genetics
MH  - Metamorphosis, Biological/genetics
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Organ Specificity
MH  - *Promoter Regions, Genetic
OTO - NOTNLM
OT  - Bombyx mori
OT  - Cathepsin B promoter
OT  - Dual-luciferase expression system
OT  - Regulatory elements
EDAT- 2014/03/19 06:00
MHDA- 2014/06/24 06:00
CRDT- 2014/03/18 06:00
PHST- 2014/02/07 00:00 [received]
PHST- 2014/03/06 00:00 [accepted]
PHST- 2014/03/18 06:00 [entrez]
PHST- 2014/03/19 06:00 [pubmed]
PHST- 2014/06/24 06:00 [medline]
AID - S0378-1119(14)00290-X [pii]
AID - 10.1016/j.gene.2014.03.012 [doi]
PST - ppublish
SO  - Gene. 2014 Jun 1;542(2):232-9. doi: 10.1016/j.gene.2014.03.012. Epub 2014 Mar 11.