PMID- 24631631
OWN - NLM
STAT- MEDLINE
DCOM- 20141218
LR  - 20140402
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 59
IP  - 5
DP  - 2014 May
TI  - The expression of MCP-1 and CCR2 in induced rats periapical lesions.
PG  - 492-9
LID - S0003-9969(14)00031-4 [pii]
LID - 10.1016/j.archoralbio.2014.02.008 [doi]
AB  - OBJECTIVE: Monocyte chemotatic protein-1 (MCP-1) has been found to promote 
      chemotaxis, differentiation, and activation of osteoclasts. The aim of this study 
      is to detect and localize the expressions of MCP-1 and its receptor CCR2 in a rat 
      model of apical periodontitis. DESIGN: After exposing the pulp of the first 
      mandibular molars, 25 Wistar rats were killed on days 0, 7, 14, 21, 28, and 35. 
      Rat jaws containing the first molar were obtained and routinely prepared for 
      histological analysis, immunohistochemistry, enzyme histochemistry and 
      immunofluorescence staining. RESULTS: Lesions expanded from day 0 to day 28, and 
      stabilized thereafter. Most of the MCP-1-positive cells were inflammatory cells, 
      and their amount increased from day 7 to day 28. Aside from inflammatory cells, 
      CCR2 was also detected in osteoclasts in the lesions. From day 7 to day 14, the 
      number of osteoclasts increased. From day 21 to day 35, fewer osteoclasts could 
      be observed, and the number of osteoclast was negatively correlated with positive 
      MCP-1 expression. CONCLUSION: These findings showed that MCP-1/CCR2 could be 
      observed and might possibly be involved in the development of periapical lesions.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Liu, Lingshuang
AU  - Liu L
AD  - School and Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Wang, Li
AU  - Wang L
AD  - School and Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Wu, Yan
AU  - Wu Y
AD  - School and Hospital of Stomatology, Wuhan University, Wuhan, China.
FAU - Peng, Bin
AU  - Peng B
AD  - School and Hospital of Stomatology, Wuhan University, Wuhan, China; School and 
      Hospital of Stomatology, Department of Conservative Dentistry and Endodontics, 
      Wuhan University, Wuhan, China. Electronic address: phs301@vip.163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140222
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Chemokine CCL2/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Immunohistochemistry
MH  - Male
MH  - Periapical Periodontitis/*metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, CCR2/*metabolism
OTO - NOTNLM
OT  - CCR2
OT  - MCP-1
OT  - Osteoclast
OT  - Periapical lesion
EDAT- 2014/03/19 06:00
MHDA- 2014/12/19 06:00
CRDT- 2014/03/18 06:00
PHST- 2013/09/02 00:00 [received]
PHST- 2014/01/26 00:00 [revised]
PHST- 2014/02/13 00:00 [accepted]
PHST- 2014/03/18 06:00 [entrez]
PHST- 2014/03/19 06:00 [pubmed]
PHST- 2014/12/19 06:00 [medline]
AID - S0003-9969(14)00031-4 [pii]
AID - 10.1016/j.archoralbio.2014.02.008 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2014 May;59(5):492-9. doi: 10.1016/j.archoralbio.2014.02.008. 
      Epub 2014 Feb 22.