PMID- 24633084
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140317
LR  - 20240413
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 6
IP  - 1
DP  - 2014 Mar 13
TI  - Recent advances in the molecular characterization of circulating tumor cells.
PG  - 595-624
LID - 10.3390/cancers6010595 [doi]
AB  - Although circulating tumor cells (CTCs) were first observed over a century ago, 
      lack of sensitive methodology precluded detailed study of these cells until 
      recently. However, technological advances have now facilitated the 
      identification, enumeration, and characterization of CTCs using a variety of 
      methods. The majority of evidence supporting the use of CTCs in clinical 
      decision-making has been related to enumeration using the CellSearch(R) system and 
      correlation with prognosis. Growing evidence also suggests that CTC monitoring 
      can provide an early indication of patient treatment response based on comparison 
      of CTC levels before and after therapy. However, perhaps the greatest potential 
      that CTCs hold for oncology lies at the level of molecular characterization. 
      Clinical treatment decisions may be more effective if they are based on molecular 
      characteristics of metastatic cells rather than on those of the primary tumor 
      alone. Molecular characterization of CTCs (which can be repeatedly isolated in a 
      minimally invasive fashion) provides the opportunity for a "real-time liquid 
      biopsy" that allows assessment of genetic drift, investigation of molecular 
      disease evolution, and identification of actionable genomic characteristics. This 
      review focuses on recent advances in this area, including approaches involving 
      immunophenotyping, fluorescence in situ hybridization (FISH), multiplex RT-PCR, 
      microarray, and genomic sequencing.
FAU - Lowes, Lori E
AU  - Lowes LE
AD  - London Regional Cancer Program, London Health Sciences Centre, London, ON N6A 
      4L6, Canada. llowes@uwo.ca.
FAU - Allan, Alison L
AU  - Allan AL
AD  - London Regional Cancer Program, London Health Sciences Centre, London, ON N6A 
      4L6, Canada. alison.allan@lhsc.on.ca.
LA  - eng
PT  - Journal Article
DEP - 20140313
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC3980613
EDAT- 2014/03/19 06:00
MHDA- 2014/03/19 06:01
PMCR- 2014/03/13
CRDT- 2014/03/18 06:00
PHST- 2013/09/02 00:00 [received]
PHST- 2014/01/28 00:00 [revised]
PHST- 2014/02/20 00:00 [accepted]
PHST- 2014/03/18 06:00 [entrez]
PHST- 2014/03/19 06:00 [pubmed]
PHST- 2014/03/19 06:01 [medline]
PHST- 2014/03/13 00:00 [pmc-release]
AID - cancers6010595 [pii]
AID - cancers-06-00595 [pii]
AID - 10.3390/cancers6010595 [doi]
PST - epublish
SO  - Cancers (Basel). 2014 Mar 13;6(1):595-624. doi: 10.3390/cancers6010595.