PMID- 24634130
OWN - NLM
STAT- MEDLINE
DCOM- 20141222
LR  - 20140317
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 13
IP  - 1
DP  - 2014 Feb 20
TI  - Construction and expression of a heterologous protein in Lactococcus lactis by 
      using the nisin-controlled gene expression system: the case of the PRRSV ORF6 
      gene.
PG  - 1088-96
LID - 10.4238/2014.February.20.10 [doi]
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a 
      threat, exerting significant economic effects on the swine industry worldwide. 
      However, none of the current commercially available vaccines can completely 
      prevent respiratory infection, trans-placental transmission, pig-to-pig 
      transmission of the virus, or maintain immune protection in sows. This study 
      provides information on PRRSV and a review of available options for PRRS control 
      strategies based on its pathogenic characteristics, immune properties, and 
      biological characteristics. In this study, the nisin-controlled expression system 
      of Lactococcus lactis was selected as a vector to express the ORF6 gene of PRRSV. 
      Food-grade recombinant, L. lactis PNZ8149/NZ3900-M/PRRS, which contained the 
      lactose operon, was successfully constructed. The molecular weight of the 
      expressed recombinant protein was approximately 19 kDa. Furthermore, the 
      recombinant protein was located on the surface of L. lactis and showed 
      reactogenicity with the antibody against PRRSV. Results of this study are 
      expected to lay a theoretical foundation for development of genetically 
      engineered L. lactis mucosal vaccines and to provide information related to its 
      immune activity and adjuvant effects.
FAU - Wang, Z H
AU  - Wang ZH
AD  - Department of Animal and Veterinary Science, Chengdu Vocational College of 
      Agricultural Science and Technology, WenJiang, Sichuan, China.
FAU - Wang, Y L
AU  - Wang YL
AD  - Isotope Research Laboratory, College of Life and Basic Sciences, Sichuan 
      Agricultural University, Xin Kang, Ya'an, Sichuan, China.
FAU - Zeng, X Y
AU  - Zeng XY
AD  - Isotope Research Laboratory, College of Life and Basic Sciences, Sichuan 
      Agricultural University, Xin Kang, Ya'an, Sichuan, China 363150834@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20140220
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (Recombinant Proteins)
RN  - 1414-45-5 (Nisin)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Gene Expression/*drug effects
MH  - *Genes, Viral
MH  - Genetic Vectors/genetics/metabolism
MH  - Lactococcus lactis/*genetics/metabolism
MH  - Nisin/*pharmacology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Recombinant Proteins/genetics/metabolism
MH  - Swine/virology
EDAT- 2014/03/19 06:00
MHDA- 2014/12/23 06:00
CRDT- 2014/03/18 06:00
PHST- 2014/03/18 06:00 [entrez]
PHST- 2014/03/19 06:00 [pubmed]
PHST- 2014/12/23 06:00 [medline]
AID - gmr2985 [pii]
AID - 10.4238/2014.February.20.10 [doi]
PST - epublish
SO  - Genet Mol Res. 2014 Feb 20;13(1):1088-96. doi: 10.4238/2014.February.20.10.