PMID- 24635663
OWN - NLM
STAT- MEDLINE
DCOM- 20141209
LR  - 20140326
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 78
IP  - 1
DP  - 2014 Apr
TI  - Single-copy gene-based chromosome painting in cucumber and its application for 
      chromosome rearrangement analysis in Cucumis.
PG  - 169-79
LID - 10.1111/tpj.12453 [doi]
AB  - Chromosome painting based on fluorescence in situ hybridization (FISH) has played 
      an important role in chromosome identification and research into chromosome 
      rearrangements, diagnosis of chromosome abnormalities and evolution in human and 
      animal species. However, it has not been applied widely in plants due to the 
      large amounts of dispersed repetitive sequences in chromosomes. In the present 
      work, a chromosome painting method for single-copy gene pools in Cucumis sativus 
      was successfully developed. Gene probes with sizes above 2 kb were detected 
      consistently. A cucumber karyotype was constructed based on FISH using a cocktail 
      containing chromosome-specific gene probes. This single-copy gene-based 
      chromosome painting (ScgCP) technique was performed by PCR amplification, 
      purification, pooling, labeling and hybridization onto chromosome spreads. Gene 
      pools containing sequential genes with an interval less than 300 kb yielded 
      painting patterns on pachytene chromosomes. Seven gene pools corresponding to 
      individual chromosomes unambiguously painted each chromosome pair of C. sativus. 
      Three mis-aligned regions on chromosome 4 were identified by the painting 
      patterns. A probe pool comprising 133 genes covering the 8 Mb distal end of 
      chromosome 4 was used to evaluate the potential utility of the ScgCP technique 
      for chromosome rearrangement research through cross-species FISH in the Cucumis 
      genus. Distinct painting patterns of this region were observed in C. sativus, C. 
      melo and C. metuliferus species. A comparative chromosome map of this region was 
      constructed between cucumber and melon. With increasing sequence resources, this 
      ScgCP technique may be applied on any other sequenced species for chromosome 
      painting research.
CI  - (c) 2014 The Authors The Plant Journal (c) 2014 John Wiley & Sons Ltd.
FAU - Lou, Qunfeng
AU  - Lou Q
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
FAU - Zhang, Yunxia
AU  - Zhang Y
FAU - He, Yuhua
AU  - He Y
FAU - Li, Ji
AU  - Li J
FAU - Jia, Li
AU  - Jia L
FAU - Cheng, Chunyan
AU  - Cheng C
FAU - Guan, Wei
AU  - Guan W
FAU - Yang, Shuqiong
AU  - Yang S
FAU - Chen, Jinfeng
AU  - Chen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140312
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosome Painting/*methods
MH  - Chromosomes, Plant/*genetics
MH  - Cucumis melo/*genetics
MH  - Cucumis sativus/cytology/*genetics
MH  - Genes, Plant/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Pachytene Stage
MH  - Polymerase Chain Reaction
OTO - NOTNLM
OT  - Cucumis sativus
OT  - chromosome painting
OT  - chromosome rearrangement
OT  - fluorescence in situ hybridization
OT  - single-copy gene
OT  - technical advance
EDAT- 2014/03/19 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/03/19 06:00
PHST- 2013/09/18 00:00 [received]
PHST- 2014/01/13 00:00 [revised]
PHST- 2014/01/20 00:00 [accepted]
PHST- 2014/03/19 06:00 [entrez]
PHST- 2014/03/19 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1111/tpj.12453 [doi]
PST - ppublish
SO  - Plant J. 2014 Apr;78(1):169-79. doi: 10.1111/tpj.12453. Epub 2014 Mar 12.