PMID- 24635731
OWN - NLM
STAT- MEDLINE
DCOM- 20150528
LR  - 20140915
IS  - 1600-0609 (Electronic)
IS  - 0902-4441 (Linking)
VI  - 93
IP  - 4
DP  - 2014 Oct
TI  - Complex MLL rearrangement in non-infiltrated bone marrow in an infant with stage 
      II precursor B-lymphoblastic lymphoma.
PG  - 349-53
LID - 10.1111/ejh.12314 [doi]
AB  - PURPOSE: Precursor B-lymphoblastic lymphoma cells are indistinguishable by 
      morphology, and immune phenotype from lymphoblasts in acute leukemia which in 
      infancy is associated with MLL rearrangements and a poor prognosis. The role of 
      MLL gene deregulation in rare cases of isolated lymphoblastic lymphoma in infants 
      is obscure. We report the case of a 10-month-old child who presented with a 
      cutaneous nodule on the left foot. Histological diagnosis was precursor 
      B-lymphoblastic lymphoma. The young age of the patient motivated us to 
      investigate the presence of an MLL rearrangement. METHODS: Cytogenetic analysis 
      was performed by fluorescence in situ hybridization (FISH), and the genomic 
      fusion partner of MLL was identified by long-distance inverse (LDI-)PCR and 
      confirmed by direct PCR. RESULTS: Fluorescence in situ hybridization screening of 
      paraffin-embedded formalin-fixed tissue indeed revealed the presence of an MLL 
      rearrangement. The genomic fusion partner was identified as AF10 by DNA 
      sequencing of the MLL breakpoint region. The MLL-AF10 fusion gene was further 
      detected in cytologically normal pretreated bone marrow. Treatment was started 
      with standard four-drug induction chemotherapy. Because of the unfavorable 
      outcome associated with MLL rearrangements in infant leukemia, we intensified 
      postremission treatment according to the Interfant-06 study protocol. The child 
      is in continuous first remission 36 months after diagnosis. CONCLUSION: This is 
      the first report of submicroscopic bone marrow involvement in MLL-rearranged 
      isolated cutaneous B-cell precursor lymphoma in an infant. To prospectively 
      address the role of MLL rearrangements in extramedullary B-lymphoblastic 
      malignancies in infants, we suggest to assess both tumors and non-infiltrated 
      bone marrow for the presence of this genetic abnormality.
CI  - (c) 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Ahlmann, Martina
AU  - Ahlmann M
AD  - Pediatric Hematology and Oncology, University Children's Hospital Muenster, 
      Muenster, Germany.
FAU - Meyer, Claus
AU  - Meyer C
FAU - Marschalek, Rolf
AU  - Marschalek R
FAU - Burkhardt, Birgit
AU  - Burkhardt B
FAU - Koehler, Gabriele
AU  - Koehler G
FAU - Klapper, Wolfram
AU  - Klapper W
FAU - Juergens, Heribert
AU  - Juergens H
FAU - Rossig, Claudia
AU  - Rossig C
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140428
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
SB  - IM
MH  - Bone Marrow/*pathology
MH  - Chromosome Breakpoints
MH  - Dermis/pathology
MH  - Humans
MH  - Infant
MH  - Male
MH  - Myeloid-Lymphoid Leukemia Protein/*genetics
MH  - Neoplasm Staging
MH  - Oncogene Proteins, Fusion
MH  - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/*genetics/*pathology
MH  - *Translocation, Genetic
OTO - NOTNLM
OT  - B-lymphoblastic lymphoma
OT  - MLL rearrangement
OT  - infant ALL
EDAT- 2014/03/19 06:00
MHDA- 2015/05/29 06:00
CRDT- 2014/03/19 06:00
PHST- 2014/03/12 00:00 [accepted]
PHST- 2014/03/19 06:00 [entrez]
PHST- 2014/03/19 06:00 [pubmed]
PHST- 2015/05/29 06:00 [medline]
AID - 10.1111/ejh.12314 [doi]
PST - ppublish
SO  - Eur J Haematol. 2014 Oct;93(4):349-53. doi: 10.1111/ejh.12314. Epub 2014 Apr 28.