PMID- 24635873
OWN - NLM
STAT- MEDLINE
DCOM- 20140827
LR  - 20211021
IS  - 1471-2202 (Electronic)
IS  - 1471-2202 (Linking)
VI  - 15
DP  - 2014 Mar 17
TI  - Effect of human umbilical cord blood mesenchymal stem cell transplantation on 
      neuronal metabolites in ischemic rabbits.
PG  - 41
LID - 10.1186/1471-2202-15-41 [doi]
AB  - BACKGROUND: Because there is little research on the effects of transplanted stem 
      cells on neuronal metabolites in infarct areas, we transplanted human umbilical 
      cord blood mesenchymal stem cells (hUCB-MSCs) into cerebral ischemic rabbits and 
      examined the neuronal metabolites. RESULTS: Rabbits (n = 40) were equally divided 
      into sham, middle cerebral artery occlusion (MCAO), hUCB-MSC, and saline groups. 
      The rabbit ischemic model was established by MCAO. The effects of hUCB-MSC 
      transplantation were assessed by proton magnetic resonance spectroscopy (1H-MRS), 
      neurological severity scores (NSSs), infarct area volume, neuronal density, and 
      optical density (OD) of microtubule-associated protein 2 (MAP2)-positive cells. 
      We also evaluated complete blood cell counts(CBCs) and serum biochemical 
      parameters. NSSs in the hUCB-MSC group at 7 and 14 days after reperfusion were 
      lower than in MCAO and saline groups (p < 0.05). Compared with MCAO and saline 
      groups at 2 weeks after MCAO, the infarction volume in the hUCB-MSC group had 
      decreased remarkably (p < 0.05). Significant neuronal metabolic changes occurred 
      in the infarct area at 24 h and 2 weeks after MCAO. 1H-MRS revealed an elevation 
      in the lactate (Lac)/creatine including phosphocreatine (Cr) ratio and a decrease 
      in the N-acetylaspartate (NAA)/Cr and choline-containing phospholipids (Cho)/Cr 
      ratios at 24 h after MCAO in the MCAO group (p < 0.01). Compared with saline and 
      MCAO groups at 24 h and 2 weeks after MCAO, NAA/Cr and Cho/Cr ratios had 
      increased significantly, whereas the Lac/Cr ratio had decreased significantly in 
      the hUCB-MSC group (p < 0.01). Neuronal density and OD of MAP2-positive cells in 
      the MCAO group were significantly lower than those in the sham group, whereas the 
      neuronal density and OD of MAP2-positive cells in the hUCB-MSC group were higher 
      than those in MCAO and saline groups (p < 0.05). CBCs and biochemical parameters 
      were unchanged in the MCAO group at 24 h and 2 weeks after hUCB-MSC 
      transplantation. CONCLUSIONS: Transplanted hUCB-MSCs might ameliorate ischemic 
      damage by influencing neuronal metabolites in the infarct area, providing 
      additional evidence for neuroprotection by stem cells. No significant changes 
      were observed in CBCs or serum biochemical parameters, suggesting that 
      intravenous infusion of hUCB-MSCs is safe for rabbits in the short-term.
FAU - Guan, Ye-Ming
AU  - Guan YM
FAU - Zhu, Yao
AU  - Zhu Y
FAU - Liu, Xue-Chun
AU  - Liu XC
FAU - Huang, Hai-Li
AU  - Huang HL
FAU - Wang, Zhi-Wei
AU  - Wang ZW
FAU - Liu, Bo
AU  - Liu B
FAU - Zhu, You-Zi
AU  - Zhu YZ
FAU - Wang, Qing-Song
AU  - Wang QS
AD  - Department of Neurology, The 105th Hospital of PLA, Clinic College, Anhui Medical 
      University, Hefei 230031, China. wangqs65@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140317
PL  - England
TA  - BMC Neurosci
JT  - BMC neuroscience
JID - 100966986
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 997-55-7 (N-acetylaspartate)
RN  - MU72812GK0 (Creatine)
RN  - N91BDP6H0X (Choline)
SB  - IM
MH  - Animals
MH  - Aspartic Acid/*analogs & derivatives/metabolism
MH  - Brain/metabolism/pathology/surgery
MH  - Brain Ischemia/*metabolism/pathology/surgery
MH  - Choline/*metabolism
MH  - Cord Blood Stem Cell Transplantation/*methods
MH  - Creatine/*metabolism
MH  - Lactic Acid/*metabolism
MH  - Male
MH  - Neurons/*metabolism
MH  - Rabbits
PMC - PMC3995438
EDAT- 2014/03/19 06:00
MHDA- 2014/08/29 06:00
PMCR- 2014/03/17
CRDT- 2014/03/19 06:00
PHST- 2013/07/15 00:00 [received]
PHST- 2014/03/12 00:00 [accepted]
PHST- 2014/03/19 06:00 [entrez]
PHST- 2014/03/19 06:00 [pubmed]
PHST- 2014/08/29 06:00 [medline]
PHST- 2014/03/17 00:00 [pmc-release]
AID - 1471-2202-15-41 [pii]
AID - 10.1186/1471-2202-15-41 [doi]
PST - epublish
SO  - BMC Neurosci. 2014 Mar 17;15:41. doi: 10.1186/1471-2202-15-41.