PMID- 24639691
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140318
LR  - 20211021
IS  - 1680-6433 (Print)
IS  - 2008-2177 (Electronic)
IS  - 1680-6433 (Linking)
VI  - 11
IP  - 1
DP  - 2013 Jan
TI  - Congenital abnormality effect of methamphetamine on histological, cellular and 
      chromosomal defects in fetal mice.
PG  - 39-46
AB  - BACKGROUND: Methamphetamine (MA) is a potent psychomotor stimulant with high 
      abuse and addictive potential. MA is a neurotoxic drug which is widely abused by 
      females of childbearing age, raising serious public health concerns in terms of 
      exposure of the fetus to the drug. Neurotoxic effects of MA on adult are well 
      known, such as dopaminergic nerve terminal degeneration and cell death in regions 
      of brain in some doses. OBJECTIVE: In the present study, we examined effect of 
      prenatal MA exposure on mouse fetuses. MATERIALS AND METHODS: In this study, 
      forty 8-12 week-old NMRI female mice were used which were mated with male mice in 
      serial days. When sperm plug was observed it was designated as gestational day 
      (GD) 0. Pregnant mice were individually housed in plastic cages. Pregnant mice 
      were divided into four groups: in first group 10 mg/kg /day MA, in second group 5 
      mg/kg /day MA and in third group saline were injected subcutaneously from GD 6 to 
      GD 14, corresponding to organogenesis period, while fourth or control group were 
      without injection. On GD 14 fetuses were removed and accomplished chromosome 
      preparation from fetal liver. Then fetal were fixed in formalin for brain 
      hematoxilin and eosine staining and TUNEL assay. RESULTS: We observed 
      morphological abnormality including exencephal fetus in 5mg/kg MA group and 
      premature fetuses in 10 mg/kg MA group. Also brain histological study showed 
      subarachnoid hemorrhage in fetal brain in both experimental groups. Fetal liver 
      karyotyping analysis was normal in fetuses of all groups and TUNEL assay in fetal 
      striatum did not show significant difference in number of apoptotic cells between 
      groups. CONCLUSION: From our results, it could be concluded that chronic abuse of 
      MA by pregnant females during organogenesis period can cause teratogenic effect 
      and brain hemorrage in fetus.
FAU - Mirjalili, Tahereh
AU  - Mirjalili T
AD  - Developmental Biology, Animal Sciences, Faculty of Biological Sciences, Shahid 
      Beheshti University, G.C., Tehran, Iran.
FAU - Kalantar, Seyed Mehdi
AU  - Kalantar SM
AD  - Department of Medical Genetics, Research and Clinical Center for Infertility, 
      Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
FAU - Shams Lahijani, Maryam
AU  - Shams Lahijani M
AD  - Developmental Biology, Animal Sciences, Faculty of Biological Sciences, Shahid 
      Beheshti University, G.C., Tehran, Iran.
FAU - Sheikhha, Mohamad Hasan
AU  - Sheikhha MH
AD  - Developmental Biology, Animal Sciences, Faculty of Biological Sciences, Shahid 
      Beheshti University, G.C., Tehran, Iran.
FAU - Talebi, Alireza
AU  - Talebi A
AD  - Department of Andrology, Research and Clinical Center for Infertility, Shahid 
      Sadoughi University of Medical Sciences, Yazd, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Reprod Med
JT  - Iranian journal of reproductive medicine
JID - 101463749
PMC - PMC3941384
OTO - NOTNLM
OT  - Abnormality
OT  - Apoptosis
OT  - Brain
OT  - Fetal
OT  - Histology
OT  - Karyotype
OT  - Methamphetamine
EDAT- 2013/01/01 00:00
MHDA- 2013/01/01 00:01
PMCR- 2013/01/01
CRDT- 2014/03/19 06:00
PHST- 2010/11/07 00:00 [received]
PHST- 2011/07/10 00:00 [revised]
PHST- 2012/02/06 00:00 [accepted]
PHST- 2014/03/19 06:00 [entrez]
PHST- 2013/01/01 00:00 [pubmed]
PHST- 2013/01/01 00:01 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - ijrm-11-039 [pii]
PST - ppublish
SO  - Iran J Reprod Med. 2013 Jan;11(1):39-46.