PMID- 24640558
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20140319
IS  - 1122-6714 (Print)
IS  - 1122-6714 (Linking)
VI  - 118
IP  - 1 Suppl
DP  - 2013
TI  - Development of human cells with RXFP1 knockdown using retroviral delivery of 
      microRNA against human RXFP1.
PG  - 10-2
AB  - To study the specific actions of relaxin through RXFP1 in human cells, it would 
      be advantageous to develop cell populations with permanent RXFP1 knockdown (KD). 
      We have developed and assessed four microRNA against human RXFP1. One of the four 
      designed microRNA displayed significant RXFP1 KD as assessed by reduced relaxin 
      binding when co-transfected with human RXFP1 into HEK-293T cells. The selected 
      microRNA sequence was subsequently retrovirally delivered into the human dermal 
      fibroblast cell line BJ3 which natively expresses RXFP1. The RXFP1 KD BJ3 cells 
      displayed diminished RXFP1 mRNA expression and complete loss of ability of 
      relaxin treatment to reduce collagen deposition after TGF-beta1 stimulation. The 
      retroviral expression of miRNA to successfully silence RXFP1 expression is an 
      invaluable tool to investigate receptor specificity, signalling and possible 
      off-target effects of newly developed relaxin analogs.
FAU - Yong, K L
AU  - Yong KL
FAU - Callander, G E
AU  - Callander GE
FAU - Bergin, R
AU  - Bergin R
FAU - Samuel, C S
AU  - Samuel CS
FAU - Bathgate, R A D
AU  - Bathgate RA
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Ital J Anat Embryol
JT  - Italian journal of anatomy and embryology = Archivio italiano di anatomia ed 
      embriologia
JID - 9612303
RN  - 0 (MicroRNAs)
RN  - 0 (RXFP1 protein, human)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Peptide)
SB  - IM
MH  - Fibroblasts/cytology/physiology
MH  - Gene Knockdown Techniques
MH  - Genetic Vectors/genetics
MH  - HEK293 Cells
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Receptors, G-Protein-Coupled/*genetics
MH  - Receptors, Peptide/*genetics
MH  - Retroviridae/*genetics
EDAT- 2013/01/01 00:00
MHDA- 2014/05/03 06:00
CRDT- 2014/03/20 06:00
PHST- 2014/03/20 06:00 [entrez]
PHST- 2013/01/01 00:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
PST - ppublish
SO  - Ital J Anat Embryol. 2013;118(1 Suppl):10-2.