PMID- 24644005
OWN - NLM
STAT- MEDLINE
DCOM- 20140909
LR  - 20160511
IS  - 2326-5205 (Electronic)
IS  - 2326-5191 (Linking)
VI  - 66
IP  - 7
DP  - 2014 Jul
TI  - Intravenous immunoglobulin attenuates experimental autoimmune arthritis by 
      inducing reciprocal regulation of Th17 and Treg cells in an 
      interleukin-10-dependent manner.
PG  - 1768-78
LID - 10.1002/art.38627 [doi]
AB  - OBJECTIVE: Intravenous immunoglobulin (IVIG) is used as a therapeutic agent in 
      various autoimmune diseases. The aims of this study were to investigate the 
      therapeutic effects of IVIG on collagen-induced arthritis (CIA) and identify the 
      mechanism responsible for any therapeutic effects. METHODS: IVIG was administered 
      to mice with CIA, and the in vivo effects were determined. Th17 and Treg cell 
      frequencies were analyzed by flow cytometry, and cytokine levels in the 
      supernatant were measured by enzyme-linked immunosorbent assay. Subpopulations of 
      T cells and B cells in the spleen were assessed by confocal microscopy. RESULTS: 
      The arthritis severity score and incidence of arthritis were lower in mice 
      treated with IVIG compared with untreated mice. Histopathologic analysis showed 
      less joint damage in mice treated with IVIG. The expression of proinflammatory 
      cytokines, specific type II collagen antibodies, and osteoclast markers was 
      significantly reduced in mice treated with IVIG. Administration of IVIG induced 
      increased FoxP3 expression and inhibited Th17 cell development. The number of 
      FoxP3+ Treg cells was increased, and the number of Th17 cells was decreased in 
      the spleens of mice treated with IVIG. The number of FoxP3+ follicular helper T 
      cells was increased, and subsequent maturation of germinal center B cells was 
      inhibited by IVIG. In addition, IVIG up-regulated interleukin-10 (IL-10) and Fcgamma 
      receptor IIB expression. The treatment effects of IVIG on arthritis were lost in 
      IL-10-knockout mice. CONCLUSION: These results showed that IVIG has therapeutic 
      effects by modulating CD4+ T cell differentiation. The therapeutic effects of 
      IVIG are dependent on IL-10.
CI  - Copyright (c) 2014 by the American College of Rheumatology.
FAU - Lee, Seon-Yeong
AU  - Lee SY
AD  - Catholic University of Korea, Seoul, South Korea.
FAU - Jung, Young-Ok
AU  - Jung YO
FAU - Ryu, Jun-Geol
AU  - Ryu JG
FAU - Kang, Chang-Min
AU  - Kang CM
FAU - Kim, Eun-Kyung
AU  - Kim EK
FAU - Son, Hye-Jin
AU  - Son HJ
FAU - Yang, Eun-Ji
AU  - Yang EJ
FAU - Ju, Ji-Hyeon
AU  - Ju JH
FAU - Kang, Young-Sun
AU  - Kang YS
FAU - Park, Sung-Hwan
AU  - Park SH
FAU - Kim, Ho-Youn
AU  - Kim HY
FAU - Cho, Mi-La
AU  - Cho ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheumatol
JT  - Arthritis & rheumatology (Hoboken, N.J.)
JID - 101623795
RN  - 0 (Fcgr2b protein, mouse)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (Receptors, IgG)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/*drug therapy/immunology
MH  - Autoimmune Diseases/*drug therapy/immunology
MH  - B-Lymphocytes/cytology/immunology
MH  - Flow Cytometry
MH  - Immunoglobulins, Intravenous/*pharmacology
MH  - Interleukin-10/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred DBA
MH  - Receptors, IgG/genetics/immunology
MH  - Severity of Illness Index
MH  - T-Lymphocytes, Regulatory/cytology/*drug effects/immunology
MH  - Th17 Cells/cytology/*drug effects/immunology
MH  - Up-Regulation/drug effects/immunology
EDAT- 2014/03/20 06:00
MHDA- 2014/09/10 06:00
CRDT- 2014/03/20 06:00
PHST- 2013/05/30 00:00 [received]
PHST- 2014/03/11 00:00 [accepted]
PHST- 2014/03/20 06:00 [entrez]
PHST- 2014/03/20 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
AID - 10.1002/art.38627 [doi]
PST - ppublish
SO  - Arthritis Rheumatol. 2014 Jul;66(7):1768-78. doi: 10.1002/art.38627.