PMID- 2464501
OWN - NLM
STAT- MEDLINE
DCOM- 19890321
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 180
IP  - 2
DP  - 1989 Feb
TI  - Thermal stability of nucleosomes studied in situ by flow cytometry: effect of 
      ionic strength and n-butyrate.
PG  - 551-6
AB  - Heating of cells permeabilized with ethanol and resuspended in aqueous media 
      increases accessibility of DNA to intercalating dyes such as acridine orange 
      (AO). The curves, representing increase in binding of AO as a function of rise in 
      temperature, indicate that the transitions are cooperative. The transitions are 
      sensitive to ionic strength and occur at lower temperatures when cells are 
      suspended in media of increasing ionic strength. Extraction of histones raises 
      accessibility of DNA to intercalators at room temperature, and heating has little 
      effect on additional binding. The results are interpreted as indicating thermal 
      destruction of nucleosomal structure in nuclear chromatin; dissociation of DNA 
      from core histones results in its increasing ability to intercalate AO, most 
      likely due to increased topological freedom to undergo unwinding and elongation 
      following binding of the intercalator. Preincubation of cells with n-butyrate, 
      known to induce histone hyperacetylation, lowers the heat stability of 
      nucleosomes by about 5 degrees C. On the other hand, no differences are observed 
      between chromatin of mitotic vs interphase cells tested over a wide range of 
      ionic strengths (0.1-0.7 N NaCl). The method appears to be useful as a probe of 
      chromatin structure at the nucleosomal level.
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
AD  - Sloan-Kettering Institute for Cancer Research, Walker Laboratory, Rye, New York 
      10580.
FAU - Carter, S P
AU  - Carter SP
LA  - eng
GR  - R 37 CA 23296/CA/NCI NIH HHS/United States
GR  - R01 CA 28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Butyrates)
RN  - 0 (Nucleosomes)
RN  - 107-92-6 (Butyric Acid)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Butyrates/*pharmacology
MH  - Butyric Acid
MH  - *Flow Cytometry
MH  - Leukemia L1210
MH  - Mice
MH  - Mitosis/drug effects
MH  - Nucleosomes/drug effects/*physiology
MH  - *Osmolar Concentration
MH  - Staining and Labeling
MH  - *Thermodynamics
EDAT- 1989/02/01 00:00
MHDA- 1989/02/01 00:01
CRDT- 1989/02/01 00:00
PHST- 1989/02/01 00:00 [pubmed]
PHST- 1989/02/01 00:01 [medline]
PHST- 1989/02/01 00:00 [entrez]
AID - 10.1016/0014-4827(89)90082-7 [doi]
PST - ppublish
SO  - Exp Cell Res. 1989 Feb;180(2):551-6. doi: 10.1016/0014-4827(89)90082-7.