PMID- 24645320
OWN - NLM
STAT- MEDLINE
DCOM- 20140408
LR  - 20181202
IS  - 1003-9279 (Print)
IS  - 1003-9279 (Linking)
VI  - 27
IP  - 5
DP  - 2013 Oct
TI  - [Relationship between serum HBV DNA level and follicular helper T lymphocyte in 
      patients with chronic hepatitis B and its significance].
PG  - 351-3
AB  - OBJECTIVE: To explore relationship between HBV DNA level and peripheral blood 
      follicular helper T lymphocyte (Tfh) in patients with chronic hepatitis B (CHB) 
      and its significance. METHODS: HBV DNA levels of 179 cases of CHB patients with 
      positive HBV DNA, positive HBeAg and positive human leukocyte antigen(HLA)-A2 
      were tested with real time fluorescent quantitative PCR. Tfh and HBV specific CTL 
      were tested with flow cytometry. IL-21 was also tested. 179 cases of CHB patients 
      were divided into group A and group B based on HBV DNA levels, 86 cases in group 
      A, HBV DNA levels were 10(4)-10(5) copies/ml, 93 cases in group B, HBV DNA levels 
      were 10(6)-10(7) copies/ml. Above testing indexes of the two groups were 
      compared. RESULTS: HBV DNA levels of group A were (4.85 +/- 0.37) log10 
      copies/ml, HBV DNA levels of group B were (6.83 +/- 0.31 ) log10 copies/ml, t = 
      27.31, P < 0. 001; Tfh of group A was (5.96 +/- 1.59)%, higher than that of group 
      B (3.71 +/- 2.15)%, t = 4.92, P < 0.01; IL-21 of group A was (42.61 +/- 
      15.11)ng/L, higher than that of group B (14.91 +/- 3.15) ng/L, t = 8.62, P < 
      0.01; HBV specific CTL of group A was (0.36 +/- 0.08)%, higher than that of group 
      B (0.18 +/- 0.06)%, t = 19.99, P < 0.001. CONCLUSION: Serum HBV DNA level of CHB 
      patients is related to the level of peripheral blood Tfh level: patients with low 
      HBV DNA level have high Tfh level, high IL-21 level and high HBV specific CTL 
      level. Patients with high HBV DNA level have low Tfh level, low IL-21 level and 
      low HBV specific CTL level. The mechanism of baseline HBV DNA level affecting 
      anti-viral therapy may be related to Tfh level.
FAU - Wang, Juan-Hua
AU  - Wang JH
FAU - Gu, Xi-Bing
AU  - Gu XB
FAU - Zhu, Yin-Fang
AU  - Zhu YF
FAU - Hua, Zhong
AU  - Hua Z
FAU - Wang, Dong
AU  - Wang D
FAU - Yang, Xiao-Juan
AU  - Yang XJ
FAU - Xu, Yue-Qin
AU  - Xu YQ
FAU - Lu, Zhong-Hua
AU  - Lu ZH
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
JT  - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang 
      bingduxue zazhi = Chinese journal of experimental and clinical virology
JID - 9602873
RN  - 0 (DNA, Viral)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Interleukins)
RN  - MKM3CA6LT1 (interleukin-21)
SB  - IM
MH  - Adult
MH  - CD4 Lymphocyte Count
MH  - DNA, Viral/*blood/genetics
MH  - Female
MH  - HLA-A2 Antigen/immunology
MH  - Hepatitis B virus/genetics/*isolation & purification
MH  - Hepatitis B, Chronic/blood/*immunology/virology
MH  - Humans
MH  - Interleukins/immunology
MH  - Male
MH  - T-Lymphocytes, Helper-Inducer/*cytology
EDAT- 2014/03/22 06:00
MHDA- 2014/04/09 06:00
CRDT- 2014/03/21 06:00
PHST- 2014/03/21 06:00 [entrez]
PHST- 2014/03/22 06:00 [pubmed]
PHST- 2014/04/09 06:00 [medline]
PST - ppublish
SO  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Oct;27(5):351-3.