PMID- 24646197
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20140730
IS  - 1532-4281 (Electronic)
IS  - 1079-9893 (Linking)
VI  - 34
IP  - 4
DP  - 2014 Aug
TI  - The effects of hydrated C(60) fullerene on gene expression profile of TRPM2 and 
      TRPM7 in hyperhomocysteinemic mice.
PG  - 317-24
LID - 10.3109/10799893.2014.896381 [doi]
AB  - BACKGROUND: Hyperhomocysteinemia (HHcy) is associated with neurodegenerative 
      diseases. Transient receptor potential melastatin (TRPM2) and TRPM7 channels may 
      be activated by oxidative stress. Hydrated C(60) fullerene (C(60)HyFn) have 
      recently gained considerable attention as promising candidates for 
      neurodegenerative states. We aimed to examine the effects on TRPM2 and TRPM7 gene 
      expression of C(60)HyFn due to marked antioxidant activity in HHcy mice. METHODS: 
      C57BL/6 J. mice were divided into four groups: (1) Control group, (2) HHcy, (3) 
      HHcy + C(60)HyFn-treated group and (4) C(60)HyFn-treated group. TRPM2 and TRPM7 
      gene expression in brains of mice were detected by real-time PCR, Western 
      blotting and immunohistochemistry. Apoptosis in brain were assessed by TUNEL 
      staining. RESULTS: mRNA expression levels of TRPM2 were significantly increased 
      in HHcy group compared to the control group. C(60)HyFn administration 
      significantly decreased serum levels of homocysteine and TRPM2 mRNA levels in 
      HHcy + C(60)HyFn group. Whereas, HHcy-treatment and C(60)HyFn administration did 
      not change the expression of TRPM7. CONCLUSION: Administration of C(60)HyFn in 
      HHcy mice significantly reduces serum homocysteine level, neuronal apoptosis and 
      expression level of TRPM2 gene. Increased expression level of TRPM2 induced by 
      oxidative stress might be involved in the ethiopathogenesis of HHcy related 
      neurologic diseases.
FAU - Etem, Ebru Onalan
AU  - Etem EO
AD  - Department of Medical Biology, Faculty of Medicine, Firat University , Elazig , 
      Turkey .
FAU - Bal, Ramazan
AU  - Bal R
FAU - Akagac, Arzu Etem
AU  - Akagac AE
FAU - Kuloglu, Tuncay
AU  - Kuloglu T
FAU - Tuzcu, Mehmet
AU  - Tuzcu M
FAU - Andrievsky, Grigory V
AU  - Andrievsky GV
FAU - Buran, Ilay
AU  - Buran I
FAU - Nedzvetsky, Victor S
AU  - Nedzvetsky VS
FAU - Baydas, Giyasettin
AU  - Baydas G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140319
PL  - England
TA  - J Recept Signal Transduct Res
JT  - Journal of receptor and signal transduction research
JID - 9509432
RN  - 0 (Fullerenes)
RN  - 0 (RNA, Messenger)
RN  - 0 (TRPM Cation Channels)
RN  - 0 (TRPM2 protein, mouse)
RN  - EC 2.7.1.- (Trpm7 protein, mouse)
RN  - NP9U26B839 (fullerene C60)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Brain/drug effects/metabolism/pathology
MH  - Fullerenes/*administration & dosage
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Hyperhomocysteinemia/*drug therapy/genetics
MH  - Mice
MH  - Oxidative Stress/drug effects
MH  - RNA, Messenger/biosynthesis
MH  - TRPM Cation Channels/*biosynthesis
OTO - NOTNLM
OT  - hydrated C60 fullerene
OT  - hyperhomocysteinemia
OT  - transient receptor potential melastatin 2
OT  - transient receptor potential melastatin 7
EDAT- 2014/03/22 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/03/21 06:00
PHST- 2014/03/21 06:00 [entrez]
PHST- 2014/03/22 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - 10.3109/10799893.2014.896381 [doi]
PST - ppublish
SO  - J Recept Signal Transduct Res. 2014 Aug;34(4):317-24. doi: 
      10.3109/10799893.2014.896381. Epub 2014 Mar 19.