PMID- 24648936
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 2049-9434 (Print)
IS  - 2049-9442 (Electronic)
IS  - 2049-9434 (Linking)
VI  - 1
IP  - 2
DP  - 2013 Mar
TI  - Evaluation of a novel real-time fluorescent polymerase chain reaction assay for 
      high-risk human papilloma virus DNA genotypes in cytological cervical screening.
PG  - 280-284
AB  - It has been confirmed that detection of high-risk human papillomavirus (HR HPV) 
      DNA is useful in cervical cancer (CC) screening. Recently, a new real-time 
      fluorescent polymerase chain reaction (PCR) assay was developed to detect HR HPV. 
      This assay can synchronize nucleic acid amplification and testing using specific 
      primers for 13 types of HR HPV genomes, combined with specific TaqMan fluorescent 
      marker probe techniques through the fluorescence automatic PCR instrument. 
      Furthermore, it uses TaqGold DNA polymerase, which minimizes the amount of 
      non-specific amplification and increases the sensitivity of the assay. The aim of 
      this study was to evaluate the analytical and clinical performance of the 
      real-time fluorescent PCR assay in CC screening, compared to the Qiagen Hybrid 
      Capture((R)) II High-Risk HPV DNA test((R)) (HC II). In total, 1,252 cervical 
      specimens were collected from women between 19 and 71 years of age. The specimens 
      were examined with three different assays, real-time fluorescent PCR assay and HC 
      II for HR HPV detection combined with liquid-based cytology. Women with 
      cytological abnormalities or HR HPV-positive results underwent colposcopy and 
      cervical biopsy. This study demonstrated good overall agreement between HC II and 
      real-time fluorescent PCR assay (overall agreement, 92.25%; Cohen's kappa=0.814). For 
      the detection of high-grade cervical intraepithelial neoplasias (CIN) and CC, the 
      sensitivity of HC II and real-time fluorescent PCR was 94.48 and 92.82%, 
      respectively, and the negative predictive value was 98.85 and 98.54%, 
      respectively. High HR HPV infection rate of the high-grade CIN and CC group was 
      detected (P<0.05). In conclusion, real-time fluorescent PCR assay provides 
      similar results compared to the HC II test for HR HPV detection and could be used 
      in CC screening in clinic.
FAU - Cheng, Jiaoying
AU  - Cheng J
AD  - Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 
      100029, P.R. China.
FAU - Bian, Meilu
AU  - Bian M
AD  - Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 
      100029, P.R. China.
FAU - Cong, Xiao
AU  - Cong X
AD  - Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 
      100029, P.R. China.
FAU - Sun, Aiping
AU  - Sun A
AD  - Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 
      100029, P.R. China.
FAU - Li, Min
AU  - Li M
AD  - Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 
      100029, P.R. China.
FAU - Ma, Li
AU  - Ma L
AD  - Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 
      100029, P.R. China.
FAU - Chen, Ying
AU  - Chen Y
AD  - Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 
      100029, P.R. China.
FAU - Liu, Jun
AU  - Liu J
AD  - Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Beijing 
      100029, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20121217
PL  - England
TA  - Biomed Rep
JT  - Biomedical reports
JID - 101613227
PMC - PMC3956221
OTO - NOTNLM
OT  - cervical cancer
OT  - human papillomavirus
OT  - hybrid capture II
OT  - real-time fluorescent polymerase chain reaction
EDAT- 2014/03/22 06:00
MHDA- 2014/03/22 06:01
PMCR- 2012/12/17
CRDT- 2014/03/21 06:00
PHST- 2012/09/17 00:00 [received]
PHST- 2012/10/18 00:00 [accepted]
PHST- 2014/03/21 06:00 [entrez]
PHST- 2014/03/22 06:00 [pubmed]
PHST- 2014/03/22 06:01 [medline]
PHST- 2012/12/17 00:00 [pmc-release]
AID - br-01-02-0280 [pii]
AID - 10.3892/br.2012.49 [doi]
PST - ppublish
SO  - Biomed Rep. 2013 Mar;1(2):280-284. doi: 10.3892/br.2012.49. Epub 2012 Dec 17.