PMID- 24648991
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 2049-9434 (Print)
IS  - 2049-9442 (Electronic)
IS  - 2049-9434 (Linking)
VI  - 1
IP  - 4
DP  - 2013 Jul
TI  - Antibiotic susceptibility and genotype patterns of Pseudomonas aeruginosa from 
      mechanical ventilation-associated pneumonia in intensive care units.
PG  - 589-593
AB  - Pseudomonas aeruginosa (P. aeruginosa) is a leading cause of morbidity and 
      mortality in patients with ventilation-associated pneumonia (VAP). It is 
      difficult to treat this infection due to acquired resistance to various 
      antibiotics. In order to understand the potential route of transmission, it is 
      important to have detailed knowledge of the genotypes and antibiotic 
      susceptibility of P. aeruginosa. The aim of this study was to determine 
      antibiotic susceptibility using the broth microdilution minimum inhibitory 
      concentration (MIC) method and to apply the Randomly Amplified Polymorphic DNA 
      (RAPD) typing method for VAP caused by P. aeruginosa in 16 patients (8 men and 8 
      women; average age at inclusion, 67.8 years; range, 53-76 years). To determine 
      antibiotic susceptibility, imipenem (IPM), cefepime (FEP) and meropenem (MEM) 
      were administered for the treatment of P. aeruginosa, yielding an effectiveness 
      of 75, 62.5, and 62.5%, respectively. According to the National Committee for 
      Clinical Laboratory Standards (NCCLS) breakpoints, 8 (50%) of the 16 mechanical 
      ventilation (MV) isolates were resistant to ceftazidime (CAZ) and pipenacillin 
      (PIP). Amikacin (AK) and aztreonam (AZT) were not as effective against P. 
      aeruginosa (75%). In addition, P. aeruginosa was completely resistant to 
      ciprofloxacin (CIP). The MV isolates were susceptible to polymyxin B (PB). RAPD 
      analysis revealed 12 genotypes for all the isolated P. aeruginosa, separated into 
      4 patterns. The results demonstrated a high incidence of P. aeruginosa isolated 
      from VAP, with endogenous and cross infections being potential reasons for P. 
      aeruginosa isolated from VAP in the intensive care units.
FAU - Xiao, Hui
AU  - Xiao H
AD  - Department of Laboratory Medicine, Shanghai First People's Hospital, Medical 
      College, Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
FAU - Ye, Xiong
AU  - Ye X
AD  - Department of Respiratory Medicine, PuDong Hospital, Shanghai 200319, P.R. China.
FAU - Liu, Qingzhong
AU  - Liu Q
AD  - Department of Laboratory Medicine, Shanghai First People's Hospital, Medical 
      College, Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
FAU - Li, Li
AU  - Li L
AD  - Department of Laboratory Medicine, Shanghai First People's Hospital, Medical 
      College, Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20130416
PL  - England
TA  - Biomed Rep
JT  - Biomedical reports
JID - 101613227
PMC - PMC3917074
OTO - NOTNLM
OT  - Pseudomonas aeruginosa
OT  - antibiotic susceptibility
OT  - genotyping
OT  - ventilation associated pneumonia
EDAT- 2014/03/22 06:00
MHDA- 2014/03/22 06:01
PMCR- 2013/04/16
CRDT- 2014/03/21 06:00
PHST- 2013/02/13 00:00 [received]
PHST- 2013/03/11 00:00 [accepted]
PHST- 2014/03/21 06:00 [entrez]
PHST- 2014/03/22 06:00 [pubmed]
PHST- 2014/03/22 06:01 [medline]
PHST- 2013/04/16 00:00 [pmc-release]
AID - br-01-04-0589 [pii]
AID - 10.3892/br.2013.94 [doi]
PST - ppublish
SO  - Biomed Rep. 2013 Jul;1(4):589-593. doi: 10.3892/br.2013.94. Epub 2013 Apr 16.