PMID- 24652327
OWN - NLM
STAT- MEDLINE
DCOM- 20150302
LR  - 20211025
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 47
IP  - 4
DP  - 2014 Apr
TI  - Delivery of the Sox9 gene promotes chondrogenic differentiation of human 
      umbilical cord blood-derived mesenchymal stem cells in an in vitro model.
PG  - 279-86
LID - S0100-879X2014005033539 [pii]
LID - 10.1590/1414-431X20133539 [doi]
AB  - SRY-related high-mobility-group box 9 (Sox9) gene is a cartilage-specific 
      transcription factor that plays essential roles in chondrocyte differentiation 
      and cartilage formation. The aim of this study was to investigate the feasibility 
      of genetic delivery of Sox9 to enhance chondrogenic differentiation of human 
      umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs). After they were 
      isolated from human umbilical cord blood within 24 h after delivery of neonates, 
      hUC-MSCs were untreated or transfected with a human Sox9-expressing plasmid or an 
      empty vector. The cells were assessed for morphology and chondrogenic 
      differentiation. The isolated cells with a fibroblast-like morphology in 
      monolayer culture were positive for the MSC markers CD44, CD105, CD73, and CD90, 
      but negative for the differentiation markers CD34, CD45, CD19, CD14, or major 
      histocompatibility complex class II. Sox9 overexpression induced accumulation of 
      sulfated proteoglycans, without altering the cellular morphology. 
      Immunocytochemistry demonstrated that genetic delivery of Sox9 markedly enhanced 
      the expression of aggrecan and type II collagen in hUC-MSCs compared with empty 
      vector-transfected counterparts. Reverse transcription-polymerase chain reaction 
      analysis further confirmed the elevation of aggrecan and type II collagen at the 
      mRNA level in Sox9-transfected cells. Taken together, short-term Sox9 
      overexpression facilitates chondrogenesis of hUC-MSCs and may thus have potential 
      implications in cartilage tissue engineering.
FAU - Wang, Z H
AU  - Wang ZH
AD  - Department of Otolaryngology - Head and Neck Surgery, The Second Hospital, Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Li, X L
AU  - Li XL
AD  - Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an, 
      China.
FAU - He, X J
AU  - He XJ
AD  - Department of Orthopedics, The Second Hospital, Xi'an Jiaotong University, Xi'an, 
      China.
FAU - Wu, B J
AU  - Wu BJ
AD  - Department of Otolaryngology - Head and Neck Surgery, The Second Hospital, Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Xu, M
AU  - Xu M
AD  - Department of Otolaryngology - Head and Neck Surgery, The Second Hospital, Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Chang, H M
AU  - Chang HM
AD  - Department of Otolaryngology - Head and Neck Surgery, Affiliated Hospital of 
      Xi'an Medical University, Xi'an, China.
FAU - Zhang, X H
AU  - Zhang XH
AD  - Department of Otolaryngology - Head and Neck Surgery, The Second Hospital, Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Xing, Z
AU  - Xing Z
AD  - Department of Clinical Dentistry, Faculty of Dentistry, Center for Clinical 
      Dental Research, University of Bergen, Bergen, Norway.
FAU - Jing, X H
AU  - Jing XH
AD  - Department of Otolaryngology - Head and Neck Surgery, The Second Hospital, Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Kong, D M
AU  - Kong DM
AD  - Department of Otolaryngology - Head and Neck Surgery, The Second Hospital, Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Kou, X H
AU  - Kou XH
AD  - Department of Otolaryngology - Head and Neck Surgery, The Second Hospital, Xi'an 
      Jiaotong University, Xi'an, China.
FAU - Yang, Y Y
AU  - Yang YY
AD  - Department of Otolaryngology - Head and Neck Surgery, The Second Hospital, Xi'an 
      Jiaotong University, Xi'an, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140318
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Aggrecans)
RN  - 0 (Collagen Type II)
RN  - 0 (SOX9 Transcription Factor)
RN  - 0 (SOX9 protein, human)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Aggrecans/biosynthesis
MH  - Blotting, Western
MH  - Cartilage/metabolism
MH  - Cell Differentiation/*genetics
MH  - Cell Proliferation/genetics
MH  - Chondrocytes/metabolism
MH  - Chondrogenesis/*genetics
MH  - Collagen Type II/biosynthesis
MH  - Fetal Blood/*cytology
MH  - Flow Cytometry
MH  - Gene Expression Regulation/physiology
MH  - Green Fluorescent Proteins
MH  - Human Umbilical Vein Endothelial Cells/cytology
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Mesenchymal Stem Cells/*cytology
MH  - Primary Cell Culture
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - SOX9 Transcription Factor/*genetics
MH  - Tissue Engineering
MH  - Transfection
PMC - PMC4075291
EDAT- 2014/03/22 06:00
MHDA- 2015/03/03 06:00
PMCR- 2014/03/18
CRDT- 2014/03/22 06:00
PHST- 2013/10/01 00:00 [received]
PHST- 2013/12/11 00:00 [accepted]
PHST- 2014/03/22 06:00 [entrez]
PHST- 2014/03/22 06:00 [pubmed]
PHST- 2015/03/03 06:00 [medline]
PHST- 2014/03/18 00:00 [pmc-release]
AID - S0100-879X2014005033539 [pii]
AID - 10.1590/1414-431X20133539 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2014 Apr;47(4):279-86. doi: 10.1590/1414-431X20133539. Epub 
      2014 Mar 18.