PMID- 24655956
OWN - NLM
STAT- MEDLINE
DCOM- 20141209
LR  - 20140324
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 46
IP  - 2
DP  - 2014
TI  - Excellent results of immunocomplex capture fluorescence analysis-I for 
      cross-match test in renal transplantation.
PG  - 332-5
LID - S0041-1345(13)01370-5 [pii]
LID - 10.1016/j.transproceed.2013.11.148 [doi]
AB  - A flow cytometry cross-match (FCXM) test is the gold standard for detection of 
      human leukocyte antigen (HLA) antibodies in renal transplantation because of its 
      high sensitivity. However, this technique can produce false-positive results when 
      non-HLA antibodies or low-titer donor-specific antibodies (DSA) are detected. To 
      determine the clinical relevance of the recently introduced novel cross-match 
      test termed immunocomplex capture fluorescence analysis (ICFA), we 
      retrospectively compared the results of ICFA and FCXM, including a single-antigen 
      bead test for detection of DSA in renal transplant recipients. We found a 
      correlation of 71.4% (235/329) between the results of ICFA-I and FCXM-T, whereas 
      that between ICFA-II and FCXM-B was 41.1% (134/326). Ninety-four patients were 
      ICFA-I negative and FCXM-T positive, and 188 were ICFA-II negative and FCXM-B 
      positive, whereas 46.8% (44/94) and 61.7% (116/188) were found to be DSA-I and 
      DSA-II negative, respectively, which classified them into the non-HLA antibody 
      and low-titer DSA groups, respectively. The mean value of molecules of equivalent 
      soluble fluorochrome for DSA-I was 22,994 in the ICFA-I-positive group, which was 
      significantly higher than 2117 in the negative group (P < .0001), whereas there 
      was no significant difference for DSA-II between the ICFA-II-positive and 
      ICFA-II-negative groups. Graft survival in the ICFA-I-negative group was 
      significantly higher than that in the ICFA-I-positive group (P = .0058). Our 
      results indicate that ICFA-I does not respond to non-HLA antibodies or low-titer 
      DSA, which have influence on graft survival. Therefore, this novel hybrid test, 
      which combines cross-match testing and HLA antibody detection functions, may be 
      useful for clinical pretransplantation evaluation of renal transplantation 
      patients.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Nishimura, K
AU  - Nishimura K
AD  - Department of Kidney Transplantation Center, Hyogo Prefectural Nishinomiya 
      Hospital, Nishinomiya, Hyogo, Japan. Electronic address: nkennishi3753@nifty.com.
FAU - Hashimoto, M
AU  - Hashimoto M
AD  - Department of Kidney Transplantation Center, Hyogo Prefectural Nishinomiya 
      Hospital, Nishinomiya, Hyogo, Japan.
FAU - Kinoshita, T
AU  - Kinoshita T
AD  - Department of Kidney Transplantation Center, Hyogo Prefectural Nishinomiya 
      Hospital, Nishinomiya, Hyogo, Japan.
FAU - Shiraishi, Y
AU  - Shiraishi Y
AD  - Department of Urology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
FAU - Ueda, N
AU  - Ueda N
AD  - Department of Kidney Transplantation Center, Hyogo Prefectural Nishinomiya 
      Hospital, Nishinomiya, Hyogo, Japan.
FAU - Nakazawa, S
AU  - Nakazawa S
AD  - Department of Kidney Transplantation Center, Hyogo Prefectural Nishinomiya 
      Hospital, Nishinomiya, Hyogo, Japan.
FAU - Hirai, T
AU  - Hirai T
AD  - Department of Kidney Transplantation Center, Hyogo Prefectural Nishinomiya 
      Hospital, Nishinomiya, Hyogo, Japan.
FAU - Ishimura, T
AU  - Ishimura T
AD  - Division of Urology, Department of Surgery Related, Kobe University Graduate 
      School of Medicine, Kobe, Japan.
FAU - Kishikawa, H
AU  - Kishikawa H
AD  - Department of Kidney Transplantation Center, Hyogo Prefectural Nishinomiya 
      Hospital, Nishinomiya, Hyogo, Japan.
FAU - Nojima, M
AU  - Nojima M
AD  - Department of Urology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
FAU - Yamamoto, S
AU  - Yamamoto S
AD  - Department of Urology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
FAU - Fujisawa, M
AU  - Fujisawa M
AD  - Division of Urology, Department of Surgery Related, Kobe University Graduate 
      School of Medicine, Kobe, Japan.
FAU - Shiina, H
AU  - Shiina H
AD  - Department of Urology, Shimane University Faculty of Medicine, Izumo, Shimane, 
      Japan.
FAU - Ichikawa, Y
AU  - Ichikawa Y
AD  - Department of Kidney Transplantation Center, Hyogo Prefectural Nishinomiya 
      Hospital, Nishinomiya, Hyogo, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
SB  - IM
MH  - Fluorescence
MH  - *Histocompatibility Testing
MH  - Humans
MH  - *Kidney Transplantation
EDAT- 2014/03/25 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/03/25 06:00
PHST- 2013/09/17 00:00 [received]
PHST- 2013/11/13 00:00 [revised]
PHST- 2013/11/27 00:00 [accepted]
PHST- 2014/03/25 06:00 [entrez]
PHST- 2014/03/25 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0041-1345(13)01370-5 [pii]
AID - 10.1016/j.transproceed.2013.11.148 [doi]
PST - ppublish
SO  - Transplant Proc. 2014;46(2):332-5. doi: 10.1016/j.transproceed.2013.11.148.