PMID- 24657599
OWN - NLM
STAT- MEDLINE
DCOM- 20150210
LR  - 20201209
IS  - 1638-6183 (Electronic)
IS  - 0300-9084 (Linking)
VI  - 102
DP  - 2014 Jul
TI  - Oxidative phosphorylation in Debaryomyces hansenii: physiological uncoupling at 
      different growth phases.
PG  - 124-36
LID - S0300-9084(14)00083-2 [pii]
LID - 10.1016/j.biochi.2014.03.003 [doi]
AB  - Physiological uncoupling of mitochondrial oxidative phosphorylation (OxPhos) was 
      studied in Debaryomyces hansenii. In other species, such as Yarrowia lipolytica 
      and Saccharomyces cerevisiae, OxPhos can be uncoupled through differential 
      expression of branched respiratory chain enzymes or by opening of a mitochondrial 
      unspecific channel (ScMUC), respectively. However D. hansenii mitochondria, which 
      contain both a branched respiratory chain and a mitochondrial unspecific channel 
      (DhMUC), selectively uncouple complex I-dependent rate of oxygen consumption in 
      the stationary growth phase. The uncoupled complex I-dependent respiration was 
      only 20% of the original activity. Inhibition was not due to inactivation of 
      complex I, lack of protein expression or to differential expression of 
      alternative oxidoreductases. Furthermore, all other respiratory chain activities 
      were normal. Decrease of complex I-dependent respiration was due to NAD(+) loss 
      from the matrix, probably through an open of DhMUC. When NAD(+) was added back, 
      coupled complex I-activity was recovered. NAD(+) re-uptake was independent of 
      DhMUC opening and seemed to be catalyzed by a NAD(+)-specific transporter, which 
      was sensitive to bathophenanthroline, bromocresol purple or 
      pyridoxal-5'-phosphate as described for S. cerevisiae mitochondrial NAD(+) 
      transporters. Loss of NAD(+) from the matrix through an open MUC is proposed as 
      an additional mechanism to uncouple OxPhos.
CI  - Copyright (c) 2014 Elsevier Masson SAS. All rights reserved.
FAU - Cabrera-Orefice, Alfredo
AU  - Cabrera-Orefice A
AD  - Dept of Molecular Genetics, Instituto de Fisiologia Celular, Universidad Nacional 
      Autonoma de Mexico, Mexico City, Mexico.
FAU - Guerrero-Castillo, Sergio
AU  - Guerrero-Castillo S
AD  - Dept of Molecular Genetics, Instituto de Fisiologia Celular, Universidad Nacional 
      Autonoma de Mexico, Mexico City, Mexico.
FAU - Diaz-Ruiz, Rodrigo
AU  - Diaz-Ruiz R
AD  - Facultad de Medicina, Programa de Posgrado en Ciencias Medicas, Odontologicas y 
      de la Salud, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico.
FAU - Uribe-Carvajal, Salvador
AU  - Uribe-Carvajal S
AD  - Dept of Molecular Genetics, Instituto de Fisiologia Celular, Universidad Nacional 
      Autonoma de Mexico, Mexico City, Mexico. Electronic address: suribe@ifc.unam.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140319
PL  - France
TA  - Biochimie
JT  - Biochimie
JID - 1264604
RN  - 0U46U6E8UK (NAD)
RN  - EC 1.- (Oxidoreductases)
SB  - IM
MH  - Cell Respiration/genetics
MH  - Debaryomyces/genetics/*growth & development
MH  - Electron Transport/*genetics
MH  - Mitochondria/enzymology/genetics
MH  - NAD/metabolism
MH  - *Oxidative Phosphorylation
MH  - Oxidoreductases/biosynthesis/metabolism
MH  - Oxygen Consumption
MH  - Saccharomyces cerevisiae
OTO - NOTNLM
OT  - AOX
OT  - Debaryomyces hansenii
OT  - NAD(+) loss
OT  - NAD(+) transport
OT  - Physiological uncoupling
OT  - Respiratory complex I
EDAT- 2014/03/25 06:00
MHDA- 2015/02/11 06:00
CRDT- 2014/03/25 06:00
PHST- 2013/12/10 00:00 [received]
PHST- 2014/03/03 00:00 [accepted]
PHST- 2014/03/25 06:00 [entrez]
PHST- 2014/03/25 06:00 [pubmed]
PHST- 2015/02/11 06:00 [medline]
AID - S0300-9084(14)00083-2 [pii]
AID - 10.1016/j.biochi.2014.03.003 [doi]
PST - ppublish
SO  - Biochimie. 2014 Jul;102:124-36. doi: 10.1016/j.biochi.2014.03.003. Epub 2014 Mar 
      19.