PMID- 24657872
OWN - NLM
STAT- MEDLINE
DCOM- 20141212
LR  - 20231110
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 80
IP  - 11
DP  - 2014 Jun
TI  - Gene transfer potential of outer membrane vesicles of Acinetobacter baylyi and 
      effects of stress on vesiculation.
PG  - 3469-83
LID - 10.1128/AEM.04248-13 [doi]
AB  - Outer membrane vesicles (OMVs) are continually released from a range of bacterial 
      species. Numerous functions of OMVs, including the facilitation of horizontal 
      gene transfer (HGT) processes, have been proposed. In this study, we investigated 
      whether OMVs contribute to the transfer of plasmids between bacterial cells and 
      species using Gram-negative Acinetobacter baylyi as a model system. OMVs were 
      extracted from bacterial cultures and tested for the ability to vector gene 
      transfer into populations of Escherichia coli and A. baylyi, including naturally 
      transformation-deficient mutants of A. baylyi. Anti-double-stranded DNA 
      (anti-dsDNA) antibodies were used to determine the movement of DNA into OMVs. We 
      also determined how stress affected the level of vesiculation and the amount of 
      DNA in vesicles. OMVs were further characterized by measuring particle size 
      distribution (PSD) and zeta potential. Transmission electron microscopy (TEM) and 
      immunogold labeling were performed using anti-fluorescein isothiocyanate 
      (anti-FITC)-conjugated antibodies and anti-dsDNA antibodies to track the movement 
      of FITC-labeled and DNA-containing OMVs. Exposure to OMVs isolated from 
      plasmid-containing donor cells resulted in HGT to A. baylyi and E. coli at 
      transfer frequencies ranging from 10(-6) to 10(-8), with transfer efficiencies of 
      approximately 10(3) and 10(2) per mug of vesicular DNA, respectively. Antibiotic 
      stress was shown to affect the DNA content of OMVs as well as their hydrodynamic 
      diameter and zeta potential. Morphological observations suggest that OMVs from A. 
      baylyi interact with recipient cells in different ways, depending on the 
      recipient species. Interestingly, the PSD measurements suggest that distinct size 
      ranges of OMVs are released from A. baylyi.
FAU - Fulsundar, Shweta
AU  - Fulsundar S
AD  - Institute of Bioinformatics and Biotechnology, University of Pune, Pune, India.
FAU - Harms, Klaus
AU  - Harms K
FAU - Flaten, Goril E
AU  - Flaten GE
FAU - Johnsen, Pal J
AU  - Johnsen PJ
FAU - Chopade, Balu Ananda
AU  - Chopade BA
FAU - Nielsen, Kaare M
AU  - Nielsen KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140321
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Acinetobacter/*genetics
MH  - DNA, Bacterial/*analysis/genetics
MH  - Escherichia coli/genetics
MH  - *Gene Transfer, Horizontal
MH  - Plasmids/analysis
MH  - Secretory Vesicles/*chemistry
MH  - *Transformation, Bacterial
PMC - PMC4018862
EDAT- 2014/03/25 06:00
MHDA- 2014/12/17 06:00
PMCR- 2014/12/01
CRDT- 2014/03/25 06:00
PHST- 2014/03/25 06:00 [entrez]
PHST- 2014/03/25 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - AEM.04248-13 [pii]
AID - 04248-13 [pii]
AID - 10.1128/AEM.04248-13 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2014 Jun;80(11):3469-83. doi: 10.1128/AEM.04248-13. Epub 
      2014 Mar 21.