PMID- 24658612
OWN - NLM
STAT- MEDLINE
DCOM- 20141208
LR  - 20151119
IS  - 1757-9708 (Electronic)
IS  - 1757-9694 (Linking)
VI  - 6
IP  - 5
DP  - 2014 May
TI  - Long-term culture and functionality of pancreatic islets monitored using 
      microelectrode arrays.
PG  - 540-4
LID - 10.1039/c3ib40261d [doi]
AB  - Extracellular recording of the glucose-induced electrical activity of mouse 
      islets of Langerhans on microelectrode arrays (MEAs) is an innovative and 
      powerful tool to address beta-cell (patho-)physiology. In a dual approach we 
      tested whether this technique can detect concentration-dependent drug effects as 
      well as characterize alterations in beta-cell activity during prolonged culture. 
      First we established conditions that allow long-term investigation of beta-cell 
      function by recording electrical activity. The results provide the first 
      measurements of beta-cell membrane potential oscillations of individual murine 
      islets during long-term culture. Oscillations were recorded for up to 34 days 
      after islet isolation. Importantly, the glucose dependence of electrical activity 
      did not change over a period of one month. Thus we can follow 
      electrophysiological changes of individual islets induced by alterations in the 
      beta-cell environment over weeks. Second, we used the MEA technique to assay 
      beta-cell damage induced by oxidative stress and to evaluate appropriate 
      protection mechanisms. Oxidative stress plays a key role in the development of 
      type 2 diabetes mellitus (T2DM). Examination of the acute effects of H2O2 on 
      electrical activity showed that the oxidant reduced the electrical activity in a 
      concentration-dependent manner. The superoxide dismutase mimetic, tempol, 
      protected against the detrimental effects of H2O2. In conclusion, we demonstrated 
      that MEA recordings can be used to address disease-related mechanisms and 
      protective interventions in beta-cells. In the future, this fundamental work 
      should enable the monitoring of the electrical activity of islets of Langerhans 
      under controlled ex vivo conditions including long-term exposure to oxidative 
      stress, glucolipotoxicity, and other diabetes-inducing agents.
FAU - Schonecker, Sven
AU  - Schonecker S
AD  - NMI Natural and Medical Sciences Institute at the University of Tubingen, 
      Department of Electrophysiology, Markwiesenstrasse 55, D-72770 Reutlingen, 
      Germany.
FAU - Kraushaar, Udo
AU  - Kraushaar U
FAU - Dufer, Martina
AU  - Dufer M
FAU - Sahr, Anika
AU  - Sahr A
FAU - Hardtner, Carmen
AU  - Hardtner C
FAU - Guenther, Elke
AU  - Guenther E
FAU - Walther, Reinhard
AU  - Walther R
FAU - Lendeckel, Uwe
AU  - Lendeckel U
FAU - Barthlen, Winfried
AU  - Barthlen W
FAU - Krippeit-Drews, Peter
AU  - Krippeit-Drews P
FAU - Drews, Gisela
AU  - Drews G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Integr Biol (Camb)
JT  - Integrative biology : quantitative biosciences from nano to macro
JID - 101478378
RN  - 0 (Antioxidants)
RN  - 0 (Cyclic N-Oxides)
RN  - 0 (Spin Labels)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - IY9XDZ35W2 (Glucose)
RN  - U78ZX2F65X (tempol)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Cyclic N-Oxides/pharmacology
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Electrophysiology/methods
MH  - Glucose/*pharmacology
MH  - Hydrogen Peroxide/antagonists & inhibitors/metabolism
MH  - Islets of Langerhans/*metabolism
MH  - Membrane Potentials/*physiology
MH  - Mice, Inbred C57BL
MH  - Microelectrodes
MH  - Oxidative Stress/*physiology
MH  - Spin Labels
EDAT- 2014/03/25 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/03/25 06:00
PHST- 2014/03/25 06:00 [entrez]
PHST- 2014/03/25 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1039/c3ib40261d [doi]
PST - ppublish
SO  - Integr Biol (Camb). 2014 May;6(5):540-4. doi: 10.1039/c3ib40261d.