PMID- 24659226
OWN - NLM
STAT- MEDLINE
DCOM- 20140903
LR  - 20211021
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 465
IP  - 1
DP  - 2014 Jul
TI  - Atypical spindle cell lipoma: a clinicopathologic, immunohistochemical, and 
      molecular study emphasizing its relationship to classical spindle cell lipoma.
PG  - 97-108
AB  - We studied a series of spindle cell lipomas arising in atypical sites and showing 
      unusual morphologic features (which we called atypical spindle cell lipoma) to 
      assess if these lesions have the same chromosomal alterations as classical 
      spindle cell lipoma but different from those found in atypical lipomatous 
      tumor/well-differentiated liposarcoma. We investigated alterations of different 
      genes in the 13q14 region and the amplification status of the MDM2 and CDK4 genes 
      at 12q14-15 by multiplex ligation-dependent probe amplification (MLPA) and 
      fluorescence in situ hybridization (FISH) analysis. In the atypical spindle cell 
      lipomas, MLPA revealed deletions in the two nearest flanking genes of RB1 (ITM2B 
      and RCBTB2) and in multiple important exons of RB1. In contrast, in classical 
      spindle cell lipomas, a less complex loss of RB1 exons was found but no deletion 
      of ITM2B and RCBTB2. Moreover, MLPA identified a deletion of the DLEU1 gene, a 
      finding which has not been reported earlier. We propose an immunohistochemical 
      panel for lipomatous tumors which comprises of MDM2, CDK4, p16, Rb, which we have 
      found useful in discriminating between atypical or classical spindle cell lipomas 
      and other adipocytic neoplasms, especially atypical lipomatous 
      tumor/well-differentiated liposarcoma. Our findings strengthen the link between 
      atypical spindle cell lipoma and classical spindle cell lipoma, and differentiate 
      them from atypical lipomatous tumor/well-differentiated liposarcoma.
FAU - Creytens, David
AU  - Creytens D
FAU - van Gorp, Joost
AU  - van Gorp J
FAU - Savola, Suvi
AU  - Savola S
FAU - Ferdinande, Liesbeth
AU  - Ferdinande L
FAU - Mentzel, Thomas
AU  - Mentzel T
FAU - Libbrecht, Louis
AU  - Libbrecht L
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Retinoblastoma Protein)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
RN  - EC 2.7.11.22 (CDK4 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 4)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*analysis/genetics
MH  - Cyclin-Dependent Kinase 4/genetics
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lipoma/genetics/*pathology
MH  - Liposarcoma/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Multiplex Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-mdm2/genetics
MH  - Retinoblastoma Protein/genetics
MH  - Skin Neoplasms/genetics/pathology
MH  - Soft Tissue Neoplasms/genetics/*pathology
EDAT- 2014/03/25 06:00
MHDA- 2014/09/04 06:00
CRDT- 2014/03/25 06:00
PHST- 2013/10/20 00:00 [received]
PHST- 2014/03/06 00:00 [accepted]
PHST- 2014/01/22 00:00 [revised]
PHST- 2014/03/25 06:00 [entrez]
PHST- 2014/03/25 06:00 [pubmed]
PHST- 2014/09/04 06:00 [medline]
AID - 10.1007/s00428-014-1568-8 [doi]
PST - ppublish
SO  - Virchows Arch. 2014 Jul;465(1):97-108. doi: 10.1007/s00428-014-1568-8.