PMID- 2465924
OWN - NLM
STAT- MEDLINE
DCOM- 19890413
LR  - 20220318
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 244
IP  - 2
DP  - 1989 Feb 27
TI  - Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, 
      expression in mitogen-stimulated blood mononuclear leukocytes, and sequence 
      similarity to mouse competence gene JE.
PG  - 487-93
AB  - The purpose of this work was to analyze cDNA encoding human monocyte 
      chemoattractant protein-1 (MCP-1), previously isolated from glioma cell line 
      culture fluid. Screening of a cDNA library from total poly(A) RNA of glioma cell 
      line U-105MG yielded a clone that coded for the entire MCP-1. Nucleotide sequence 
      analysis and comparison with the amino acid sequence of purified MCP-1 showed 
      that the cDNA clone comprises a 53-nucleotide 5'-non-coding region, an open 
      reading frame coding for a 99-residue protein of which the last 76 residues 
      correspond exactly to pure MCP-1, and a 389-nucleotide 3'-untranslated region. 
      The hydrophobicity of the first 23 residues is typical of a signal peptide. 
      Southern blot analysis of human and animal genomic DNA showed that there is a 
      single MCP-1 gene, which is conserved in several primates. MCP-1 mRNA was induced 
      in human peripheral blood mononuclear leukocytes (PBMNLs) by PHA, LPS and IL-1, 
      but not by IL-2, TNF, or IFN-gamma. Among proteins with similar sequences, the 
      coding regions of MCP-1 and mouse JE show 68% identity. This suggest that MCP-1 
      is the human homologue of the mouse competence gene JE.
FAU - Yoshimura, T
AU  - Yoshimura T
AD  - Laboratory of Immunobiology, National Cancer Institute, Frederick, MD 21701.
FAU - Yuhki, N
AU  - Yuhki N
FAU - Moore, S K
AU  - Moore SK
FAU - Appella, E
AU  - Appella E
FAU - Lerman, M I
AU  - Lerman MI
FAU - Leonard, E J
AU  - Leonard EJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Messenger)
RN  - 24937-83-5 (Poly A)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Cell Line
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*genetics
MH  - *Chemotaxis, Leukocyte
MH  - Cloning, Molecular
MH  - DNA/blood/genetics
MH  - DNA, Neoplasm/genetics
MH  - Glioma
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Monocytes/*physiology
MH  - Poly A/genetics
MH  - RNA/genetics
MH  - RNA, Messenger
MH  - Sequence Homology, Nucleic Acid
EDAT- 1989/02/27 00:00
MHDA- 1989/02/27 00:01
CRDT- 1989/02/27 00:00
PHST- 1989/02/27 00:00 [pubmed]
PHST- 1989/02/27 00:01 [medline]
PHST- 1989/02/27 00:00 [entrez]
AID - 0014-5793(89)80590-3 [pii]
AID - 10.1016/0014-5793(89)80590-3 [doi]
PST - ppublish
SO  - FEBS Lett. 1989 Feb 27;244(2):487-93. doi: 10.1016/0014-5793(89)80590-3.