PMID- 24660043
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140324
LR  - 20211021
IS  - 1932-1058 (Print)
IS  - 1932-1058 (Electronic)
IS  - 1932-1058 (Linking)
VI  - 8
IP  - 1
DP  - 2014 Jan
TI  - Using microfluidic chip to form brain-derived neurotrophic factor concentration 
      gradient for studying neuron axon guidance.
PG  - 014108
LID - 10.1063/1.4864235 [doi]
LID - 014108
AB  - Molecular gradients play a significant role in regulating biological and 
      pathological processes. Although conventional gradient-generators have been used 
      for studying chemotaxis and axon guidance, there are still many limitations, 
      including the inability to maintain stable tempo-spatial gradients and the lack 
      of the cell monitoring in a real-time manner. To overcome these shortcomings, 
      microfluidic devices have been developed. In this study, we developed a 
      microfluidic gradient device for regulating neuron axon guidance. A microfluidic 
      device enables the generation of Brain-derived neurotrophic factor (BDNF) 
      gradient profiles in a temporal and spatial manner. We test the effect of the 
      gradient profiles on axon guidance, in the BDNF concentration gradient axon 
      towards the high concentration gradient. This microfluidic gradient device could 
      be used as a powerful tool for cell biology research.
FAU - Huang, Hui
AU  - Huang H
AD  - Department of Clinical Laboratory Science, College of Medical Laboratory, 
      Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, 
      Shapingba District, Chongqing 400038, China.
FAU - Jiang, Lili
AU  - Jiang L
AD  - Department of Clinical Laboratory Science, College of Medical Laboratory, 
      Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, 
      Shapingba District, Chongqing 400038, China.
FAU - Li, Shu
AU  - Li S
AD  - Department of Microbiology, College of Basic Medical Science, Third Military 
      Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, 
      China.
FAU - Deng, Jun
AU  - Deng J
AD  - Department of Clinical Laboratory Science, College of Medical Laboratory, 
      Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, 
      Shapingba District, Chongqing 400038, China.
FAU - Li, Yan
AU  - Li Y
AD  - Department of Clinical Laboratory Science, College of Medical Laboratory, 
      Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, 
      Shapingba District, Chongqing 400038, China.
FAU - Yao, Jie
AU  - Yao J
AD  - Department of Clinical Laboratory Science, College of Medical Laboratory, 
      Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, 
      Shapingba District, Chongqing 400038, China.
FAU - Li, Biyuan
AU  - Li B
AD  - Department of Clinical Laboratory Science, College of Medical Laboratory, 
      Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, 
      Shapingba District, Chongqing 400038, China.
FAU - Zheng, Junsong
AU  - Zheng J
AD  - Department of Clinical Laboratory Science, College of Medical Laboratory, 
      Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, 
      Shapingba District, Chongqing 400038, China.
LA  - eng
PT  - Journal Article
DEP - 20140219
PL  - United States
TA  - Biomicrofluidics
JT  - Biomicrofluidics
JID - 101293825
PMC - PMC3945791
EDAT- 2014/03/25 06:00
MHDA- 2014/03/25 06:01
PMCR- 2015/02/19
CRDT- 2014/03/25 06:00
PHST- 2013/09/18 00:00 [received]
PHST- 2014/01/24 00:00 [accepted]
PHST- 2014/03/25 06:00 [entrez]
PHST- 2014/03/25 06:00 [pubmed]
PHST- 2014/03/25 06:01 [medline]
PHST- 2015/02/19 00:00 [pmc-release]
AID - 009401BMF [pii]
AID - 10.1063/1.4864235 [doi]
PST - epublish
SO  - Biomicrofluidics. 2014 Feb 19;8(1):014108. doi: 10.1063/1.4864235. eCollection 
      2014 Jan.