PMID- 24661874
OWN - NLM
STAT- MEDLINE
DCOM- 20140624
LR  - 20140424
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 446
IP  - 4
DP  - 2014 Apr 18
TI  - Tolerance induction between two different strains of parental mice prevents 
      graft-versus-host disease in haploidentical hematopoietic stem cell 
      transplantation to F1 mice.
PG  - 1035-41
LID - S0006-291X(14)00498-7 [pii]
LID - 10.1016/j.bbrc.2014.03.055 [doi]
AB  - Haploidentical hematopoietic stem cell transplantation (Haplo-HSCT) has been 
      employed worldwide in recent years and led to favorable outcome in a group of 
      patients who do not have human leukocyte antigen (HLA)-matched donors. However, 
      the high incidence of severe graft-versus-host disease (GVHD) is a major problem 
      for Haplo-HSCT. In the current study, we performed a proof of concept mouse study 
      to test whether induction of allogeneic tolerance between two different parental 
      strains was able to attenuate GVHD in Haplo-HSCT to the F1 mice. We induced 
      alloantigen tolerance in C3H mice (H-2k) using ultraviolet B (UVB) irradiated 
      immature dendritic cells (iDCs) derived from the cultures of Balb/c bone marrow 
      cells. Then, we performed Haplo-HSCT using tolerant C3H mice as donors to F1 mice 
      (C3HxBalb/c). The results demonstrated that this approach markedly reduced 
      GVHD-associated death and significantly prolonged the survival of recipient mice 
      in contrast to the groups with donors (C3H mice) that received infusion of 
      non-UVB-irradiated DCs. Further studies showed that there were enhanced Tregs in 
      the tolerant mice and alloantigen-specific T cell response was skewed to more 
      IL-10-producing T cells, suggesting that these regulatory T cells might have 
      contributed to the attenuation of GVHD. This study suggests that it is a feasible 
      approach to preventing GVHD in Haplo-HSCT in children by pre-induction of 
      alloantigen tolerance between the two parents. This concept may also lead to more 
      opportunities in cell-based immunotherapy for GVHD post Haplo-HSCT.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Guo, Yixian
AU  - Guo Y
AD  - Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 
      100053, People's Republic of China.
FAU - Zhang, Lanfang
AU  - Zhang L
AD  - Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 
      100053, People's Republic of China.
FAU - Wan, Suigui
AU  - Wan S
AD  - Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 
      100053, People's Republic of China.
FAU - Sun, Xuejing
AU  - Sun X
AD  - Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 
      100053, People's Republic of China.
FAU - Wu, Yongxia
AU  - Wu Y
AD  - Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 
      100053, People's Republic of China.
FAU - Yu, Xue-Zhong
AU  - Yu XZ
AD  - Department of Microbiology & Immunology, Medical University of South Carolina, 
      Charleston, SC 29425, USA.
FAU - Xia, Chang-Qing
AU  - Xia CQ
AD  - Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 
      100053, People's Republic of China. Electronic address: cqx65@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140321
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology/*radiation effects/*transplantation
MH  - Graft vs Host Disease/immunology/*prevention & control
MH  - Hematopoietic Stem Cell Transplantation/*methods
MH  - Humans
MH  - Immune Tolerance
MH  - Interleukin-10/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - T-Lymphocytes, Regulatory/immunology
MH  - Ultraviolet Rays
OTO - NOTNLM
OT  - Dendritic cells
OT  - Graft-versus-host disease
OT  - Haploidentical
OT  - Hematopoietic stem cell transplantation
OT  - Immune tolerance
OT  - Regulatory T cells
EDAT- 2014/03/26 06:00
MHDA- 2014/06/25 06:00
CRDT- 2014/03/26 06:00
PHST- 2014/03/10 00:00 [received]
PHST- 2014/03/14 00:00 [accepted]
PHST- 2014/03/26 06:00 [entrez]
PHST- 2014/03/26 06:00 [pubmed]
PHST- 2014/06/25 06:00 [medline]
AID - S0006-291X(14)00498-7 [pii]
AID - 10.1016/j.bbrc.2014.03.055 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2014 Apr 18;446(4):1035-41. doi: 
      10.1016/j.bbrc.2014.03.055. Epub 2014 Mar 21.