PMID- 24664778
OWN - NLM
STAT- MEDLINE
DCOM- 20140724
LR  - 20161109
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 801
DP  - 2014
TI  - Intrascleral transplantation of a collagen sheet with cultured brain-derived 
      neurotrophic factor expressing cells partially rescues the retina from damage due 
      to acute high intraocular pressure.
PG  - 837-43
LID - 10.1007/978-1-4614-3209-8_105 [doi]
AB  - We constructed brain-derived neurotrophic factor (BDNF) expressing rat retinal 
      pigment epithelial (RPE) cells by stable transfection of BDNF cDNA, and the RPE 
      cells were cultured on a cross-linked collagen sheet (Coll-RPE-BDNF). BDNF 
      expression of the Coll-RPE-BDNF was confirmed by western blot, and the 
      Coll-RPE-BDNF was transplanted into the rabbit sclera. In vivo BDNF expression 
      was confirmed by His expression that was linked to the expressing BDNF. The 
      effect of the released BDNF was examined in a rabbit acute high intraocular 
      pressure system by electroretinogram and histological examination. Statistically 
      significant preservation of ERG b wave amplitude was observed in the rabbits 
      treated by Coll-RPE-BDNF when compared to that of no treatment. Statistically 
      significant preservation of the thickness of the inner nuclear layer at the 
      transplanted area was observed in the rabbits treated by Coll-RPE-BDNF compared 
      to that of no treatment. Intra-scleral Coll-RPE-BDNF transplantation may 
      partially rescue retinal cells from acute high intraocular pressure.
FAU - Abe, Toshiaki
AU  - Abe T
AD  - Division of Clinical Cell Therapy, Center for Advanced Medical Research and 
      Development (ART), Graduate School of Medicine, Tohoku University, Sendai, Japan, 
      toshi@oph.med.tohoku.ac.jp.
FAU - Tokita-Ishikawa, Yumi
AU  - Tokita-Ishikawa Y
FAU - Onami, Hideyuki
AU  - Onami H
FAU - Katsukura, Yuki
AU  - Katsukura Y
FAU - Kaji, Hirokazu
AU  - Kaji H
FAU - Nishizawa, Matsuhiko
AU  - Nishizawa M
FAU - Nagai, Nobuhiro
AU  - Nagai N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cross-Linking Reagents)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Transplantation/*methods
MH  - Collagen/pharmacology
MH  - Cross-Linking Reagents/pharmacology
MH  - Electroretinography
MH  - Graft Survival
MH  - Intraocular Pressure/*physiology
MH  - Male
MH  - Rabbits
MH  - Rats
MH  - Retina/cytology/*physiology
MH  - Retinal Diseases/etiology/*surgery
MH  - Retinal Pigment Epithelium/cytology/metabolism/*transplantation
MH  - Sclera/surgery
EDAT- 2014/03/26 06:00
MHDA- 2014/07/25 06:00
CRDT- 2014/03/26 06:00
PHST- 2014/03/26 06:00 [entrez]
PHST- 2014/03/26 06:00 [pubmed]
PHST- 2014/07/25 06:00 [medline]
AID - 10.1007/978-1-4614-3209-8_105 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 2014;801:837-43. doi: 10.1007/978-1-4614-3209-8_105.