PMID- 24666017
OWN - NLM
STAT- MEDLINE
DCOM- 20150202
LR  - 20181202
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Linking)
VI  - 122
IP  - 3
DP  - 2014 Jun
TI  - Lentiviral-mediated expression of SATB2 promotes osteogenic differentiation of 
      bone marrow stromal cells in vitro and in vivo.
PG  - 190-7
LID - 10.1111/eos.12122 [doi]
AB  - Special AT-rich sequence-binding protein 2 (SATB2 ) acts as a potent 
      transcription factor to promote osteoblast differentiation and bone regeneration. 
      In this study, we first used lentiviral-mediated gene transfer of Satb2 into 
      mouse bone marrow stromal cells (BMSCs) and investigated the capacity of SATB2 
      overexpression to promote osteogenic differentiation in vitro and in vivo. We 
      found that LV-Satb2 -transduced BMSCs produced SATB2 protein and underwent rapid 
      and marked osteogenic differentiation, as demonstrated by increased expression of 
      osteoblastic genes, including runt-related transcription factor 2 (Runx2), 
      transcription factor Sp7 (Sp7), activating transcription factor 4 (Atf4), and 
      bone sialoprotein (Bsp), and increased alkaline phosphatase activity and Alizarin 
      Red S staining. To analyze the induction of bone formation in vivo, 
      LV-Satb2-transduced BMSCs were implanted into the hindlimbs of syngeneic mice, 
      with beta-tricalcium phosphate as the scaffolding material. Four weeks after 
      implantation, transduction with LV-Satb2 had greatly enhanced the formation of 
      new bone. These data demonstrated the capacity of lentiviral-mediated SATB2 to 
      promote the osteogenic differentiation of BMSCs in vitro and to enhance bone 
      formation through a tissue-engineering technique that may be useful in 
      bone-regenerative medicine.
CI  - (c) 2014 Eur J Oral Sci.
FAU - Gong, Yiming
AU  - Gong Y
AD  - Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai, China.
FAU - Qian, Yanyan
AU  - Qian Y
FAU - Yang, Fei
AU  - Yang F
FAU - Wang, Huijun
AU  - Wang H
FAU - Yu, Youcheng
AU  - Yu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140326
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Atf4 protein, mouse)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Ibsp protein, mouse)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 0 (Matrix Attachment Region Binding Proteins)
RN  - 0 (Runx2 protein, mouse)
RN  - 0 (SATB2 protein, mouse)
RN  - 0 (Sp7 Transcription Factor)
RN  - 0 (Sp7 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 0 (beta-tricalcium phosphate)
RN  - 145891-90-3 (Activating Transcription Factor 4)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Activating Transcription Factor 4/analysis
MH  - Alkaline Phosphatase/analysis
MH  - Animals
MH  - Bone Regeneration/physiology
MH  - Calcium Phosphates/chemistry
MH  - Cell Culture Techniques
MH  - Cell Differentiation/genetics
MH  - Core Binding Factor Alpha 1 Subunit/analysis
MH  - Gene Transfer Techniques
MH  - Genetic Vectors/*genetics
MH  - Integrin-Binding Sialoprotein/analysis
MH  - Lentivirus/*genetics
MH  - Male
MH  - Matrix Attachment Region Binding Proteins/*genetics
MH  - Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoblasts/physiology
MH  - Osteogenesis/*genetics
MH  - Sp7 Transcription Factor
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/chemistry
MH  - Transcription Factors/analysis/*genetics
MH  - Transplantation, Isogeneic
MH  - Zinc Fingers/genetics
OTO - NOTNLM
OT  - SATB2
OT  - bone marrow stromal cell
OT  - lentivirus
OT  - osteogenic differentiation
OT  - beta-tricalcium phosphate
EDAT- 2014/03/29 06:00
MHDA- 2015/02/03 06:00
CRDT- 2014/03/27 06:00
PHST- 2014/01/23 00:00 [accepted]
PHST- 2014/03/27 06:00 [entrez]
PHST- 2014/03/29 06:00 [pubmed]
PHST- 2015/02/03 06:00 [medline]
AID - 10.1111/eos.12122 [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2014 Jun;122(3):190-7. doi: 10.1111/eos.12122. Epub 2014 Mar 26.