PMID- 24666389
OWN - NLM
STAT- MEDLINE
DCOM- 20150303
LR  - 20211021
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 40
IP  - 1
DP  - 2014 Jul
TI  - Reduced levels of brain-derived neurotrophic factor contribute to synaptic 
      imbalance during the critical period of respiratory development in rats.
PG  - 2183-95
LID - 10.1111/ejn.12568 [doi]
AB  - Previously, our electrophysiological studies revealed a transient imbalance 
      between suppressed excitation and enhanced inhibition in hypoglossal motoneurons 
      of rats on postnatal days (P) 12-13, a critical period when abrupt neurochemical, 
      metabolic, ventilatory and physiological changes occur in the respiratory system. 
      The mechanism underlying the imbalance is poorly understood. We hypothesised that 
      the imbalance was contributed by a reduced expression of brain-derived 
      neurotrophic factor (BDNF), which normally enhances excitation and suppresses 
      inhibition. We also hypothesised that exogenous BDNF would partially reverse this 
      synaptic imbalance. Immunohistochemistry/single-neuron optical densitometry, 
      real-time quantitative PCR (RT-qPCR) and whole-cell patch-clamp recordings were 
      done on hypoglossal motoneurons in brainstem slices of rats during the first 
      three postnatal weeks. Our results indicated that: (1) the levels of BDNF and its 
      high-affinity tyrosine receptor kinase B (TrkB) receptor mRNAs and proteins were 
      relatively high during the first 1-1.5 postnatal weeks, but dropped precipitously 
      at P12-13 before rising again afterwards; (2) exogenous BDNF significantly 
      increased the normally lowered frequency of spontaneous excitatory postsynaptic 
      currents but decreased the normally heightened amplitude and frequency of 
      spontaneous inhibitory postsynaptic currents (sIPSCs) during the critical period; 
      (3) exogenous BDNF also decreased the normally heightened frequency of miniature 
      IPSCs at P12-13; and (4) the effect of exogenous BDNF was partially blocked by 
      K252a, a TrkB receptor antagonist. Thus, our results are consistent with our 
      hypothesis that BDNF and TrkB play an important role in the synaptic imbalance 
      during the critical period. This may have significant implications for the 
      mechanism underlying sudden infant death syndrome.
CI  - (c) 2014 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.
FAU - Gao, Xiu-Ping
AU  - Gao XP
AD  - Department of Cell Biology, Neurobiology and Anatomy, Medical College of 
      Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI, 53226, USA.
FAU - Liu, Qiuli
AU  - Liu Q
FAU - Nair, Bindu
AU  - Nair B
FAU - Wong-Riley, Margaret T T
AU  - Wong-Riley MT
LA  - eng
GR  - R01 HD048954/HD/NICHD NIH HHS/United States
GR  - HD048954/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140326
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbazoles)
RN  - 0 (Central Nervous System Agents)
RN  - 0 (Indole Alkaloids)
RN  - 0 (RNA, Messenger)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain Stem/drug effects/*growth & development/physiology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Carbazoles/pharmacology
MH  - Central Nervous System Agents/pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects/physiology
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Hypoglossal Nerve/drug effects/*growth & development/physiology
MH  - Indole Alkaloids/pharmacology
MH  - Inhibitory Postsynaptic Potentials/drug effects/physiology
MH  - Male
MH  - Miniature Postsynaptic Potentials/drug effects/physiology
MH  - Motor Neurons/drug effects/*physiology
MH  - RNA, Messenger/metabolism
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/antagonists & inhibitors/metabolism
MH  - *Respiration/drug effects
MH  - Synapses/drug effects/*physiology
MH  - Tissue Culture Techniques
PMC - PMC4107017
MID - NIHMS572041
OTO - NOTNLM
OT  - BDNF/TrkB
OT  - critical period
OT  - immunohistochemistry
OT  - real-time quantitative PCR
OT  - whole-cell patch clamp recording
COIS- All authors have no conflict of interest to declare.
EDAT- 2014/03/29 06:00
MHDA- 2015/03/04 06:00
PMCR- 2015/07/01
CRDT- 2014/03/27 06:00
PHST- 2013/08/27 00:00 [received]
PHST- 2014/02/15 00:00 [revised]
PHST- 2014/02/18 00:00 [accepted]
PHST- 2014/03/27 06:00 [entrez]
PHST- 2014/03/29 06:00 [pubmed]
PHST- 2015/03/04 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - 10.1111/ejn.12568 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2014 Jul;40(1):2183-95. doi: 10.1111/ejn.12568. Epub 2014 Mar 26.