PMID- 24669709
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20231213
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 31
IP  - 12
DP  - 2013 Dec
TI  - [Determination of twenty free amino acids in flue-cured tobacco leaves using 
      ultra performance liquid chromatography-single quadruple mass spectrometry and 
      pre-column derivatization].
PG  - 1182-8
AB  - Free amino acids in flue-cured tobacco leaves were investigated using the ultra 
      performance liquid chromatography-single quadruple mass spectrometry detection 
      and pre-column derivatization method. The validation results showed that the 
      method could meet the analytical requirements. A total of 138 tobacco leaf 
      samples were collected from 14 provinces in China in 2011 in which the free amino 
      acids were determined. The relative standard deviations (RSDs) of the contents of 
      free amino acids in different growing regions ranged from 28.50%-94.20%, and 
      those of asparagine and glutamine were over 80%. The RSDs of the contents of free 
      amino acids in full aroma tobacco leaves were larger than those in fresh aroma 
      and medium aroma tobacco leaves. The principal component analysis (PCA) and 
      non-parameter Mann-Whitney U test were used for data analysis. The free amino 
      acids of the same aroma type grown in different regions or different aroma types 
      in the same province showed great variation. The contents of free amino acids of 
      full aroma tobacco grown in Southeast region were much lower than those in 
      Huanghuai region. The contents of free amino acids in Hunan province were much 
      lower than the average contents. The results showed that free amino acids in 
      flue-cured tobacco leaves were affected by the growing region.
FAU - Li, Haoli
AU  - Li H
FAU - Zhao, Chunxia
AU  - Zhao C
FAU - Zhang, Junjie
AU  - Zhang J
FAU - Fu, Jiajun
AU  - Fu J
FAU - Wang, Ying
AU  - Wang Y
FAU - Lu, Xin
AU  - Lu X
FAU - Xu, Guowang
AU  - Xu G
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Amino Acids)
SB  - IM
MH  - Amino Acids/*analysis
MH  - China
MH  - *Chromatography, High Pressure Liquid
MH  - *Mass Spectrometry
MH  - Plant Leaves/chemistry
MH  - Principal Component Analysis
MH  - Nicotiana/*chemistry
EDAT- 2014/03/29 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/03/28 06:00
PHST- 2014/03/28 06:00 [entrez]
PHST- 2014/03/29 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.3724/sp.j.1123.2013.08006 [doi]
PST - ppublish
SO  - Se Pu. 2013 Dec;31(12):1182-8. doi: 10.3724/sp.j.1123.2013.08006.