PMID- 24670132
OWN - NLM
STAT- MEDLINE
DCOM- 20150625
LR  - 20161020
IS  - 1439-7609 (Electronic)
IS  - 1439-7595 (Linking)
VI  - 24
IP  - 6
DP  - 2014 Nov
TI  - Application of a new immunohistology scoring system (IH score): analysis of TNF-alpha 
      in synovium related to disease activity score in infliximab-treated patients with 
      rheumatoid arthritis.
PG  - 910-4
LID - 10.3109/14397595.2014.887047 [doi]
AB  - OBJECTIVES: This study aimed to analyze the relationship between the expression 
      of tumor necrosis factor alpha (TNF-alpha) or interleukin-6 (IL-6) in synovium and 
      the disease activity score (DAS) 28 (C-reactive protein, CRP) in treatment of 
      infliximab for rheumatoid arthritis (RA). METHODS: Synovial tissues were obtained 
      from 16 infliximab-treated patients and assessed for TNF-alpha and IL-6 with a new 
      immunohistology (IH) scoring system. The validation of IH score was performed and 
      applied for the analysis of correlation between synovial TNF-alpha or IL-6 and DAS28 
      (CRP) in addition to Rooney score. RESULTS: The IH score had high internal 
      validity; the IH score of TNF-alpha strongly correlated with serum CRP and matrix 
      metalloprotease-3 (MMP-3), as well as DAS28 (CRP) and the Rooney score. IL-6 did 
      not correlate with DAS28 (CRP). CONCLUSIONS: This study indicates that the IH 
      score is useful as a new procedure to assess the cytokine expression easily and 
      TNF-alpha in synovium correlates with disease activity in patients with RA treated 
      with infliximab.
FAU - Kanbe, Katsuaki
AU  - Kanbe K
AD  - Department of Orthopaedic Surgery, Tokyo Women's Medical University , Medical 
      Center East, Tokyo , Japan.
FAU - Hara, Ryota
AU  - Hara R
FAU - Chiba, Junji
AU  - Chiba J
FAU - Inoue, Yasuo
AU  - Inoue Y
FAU - Taguchi, Masashi
AU  - Taguchi M
FAU - Tanaka, Yasuhito
AU  - Tanaka Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140326
PL  - England
TA  - Mod Rheumatol
JT  - Modern rheumatology
JID - 100959226
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antirheumatic Agents)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - B72HH48FLU (Infliximab)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Monoclonal/*therapeutic use
MH  - Antirheumatic Agents/*therapeutic use
MH  - Arthritis, Rheumatoid/drug therapy/*metabolism/pathology
MH  - C-Reactive Protein/metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Infliximab
MH  - Interleukin-6/metabolism
MH  - Male
MH  - Middle Aged
MH  - Observer Variation
MH  - Severity of Illness Index
MH  - Synovial Membrane/*metabolism/pathology
MH  - Treatment Outcome
MH  - Tumor Necrosis Factor-alpha/*metabolism
OTO - NOTNLM
OT  - Histology
OT  - Infliximab
OT  - Rheumatoid arthritis
OT  - Synovium
OT  - TNF-alpha
EDAT- 2014/03/29 06:00
MHDA- 2015/06/26 06:00
CRDT- 2014/03/28 06:00
PHST- 2014/03/28 06:00 [entrez]
PHST- 2014/03/29 06:00 [pubmed]
PHST- 2015/06/26 06:00 [medline]
AID - 10.3109/14397595.2014.887047 [doi]
PST - ppublish
SO  - Mod Rheumatol. 2014 Nov;24(6):910-4. doi: 10.3109/14397595.2014.887047. Epub 2014 
      Mar 26.