PMID- 2467188
OWN - NLM
STAT- MEDLINE
DCOM- 19890501
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 9
IP  - 1
DP  - 1989 Jan
TI  - Inherited immunodeficiency with a defect in a major histocompatibility complex 
      class II promoter-binding protein differs in the chromatin structure of the 
      HLA-DRA gene.
PG  - 296-302
AB  - A defect in a trans-regulatory factor which controls major histocompatibility 
      complex class II gene expression is responsible for an inherited form of 
      immunodeficiency with a lack of expression of human leukocyte antigen (HLA) class 
      II antigens. We have recently described and cloned an HLA class II promoter 
      DNA-binding protein, RF-X, present in normal B cells and absent in these class 
      II-deficient regulatory mutants. Here we report that these in vitro results 
      correlate with a specific change in the chromatin structure of the class II 
      promoter: two prominent DNase I-hypersensitive sites were identified in the 
      promoter of the HLA-DRA gene in normal B lymphocytes and found to be absent in 
      the class II-deficient mutant cells. The same two prominent DNase 
      I-hypersensitive sites were observed in normal fibroblastic cells induced by 
      gamma interferon to express class II genes. Interestingly, they were also 
      observed in the uninduced class II-negative fibroblastic cells, which have also 
      been shown to have a normal RF-X binding pattern. We conclude that the two DNase 
      I-hypersensitive sites in the HLA-DRA promoter reflect features in chromatin 
      structure which correlate with the binding of the trans-acting factor RF-X and 
      which are necessary but not sufficient for the expression of class II genes.
FAU - Gonczy, P
AU  - Gonczy P
AD  - Department of Microbiology, University of Geneva Medical School, Switzerland.
FAU - Reith, W
AU  - Reith W
FAU - Barras, E
AU  - Barras E
FAU - Lisowska-Grospierre, B
AU  - Lisowska-Grospierre B
FAU - Griscelli, C
AU  - Griscelli C
FAU - Hadam, M R
AU  - Hadam MR
FAU - Mach, B
AU  - Mach B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HLA-D Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 63231-63-0 (RNA)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
SB  - IM
MH  - B-Lymphocytes/metabolism
MH  - Blotting, Southern
MH  - Cell Line, Transformed
MH  - Chromatin/*analysis
MH  - DNA-Binding Proteins/*genetics
MH  - Deoxyribonuclease I
MH  - *Genes, MHC Class II
MH  - HLA-D Antigens/*genetics
MH  - Herpesvirus 4, Human/metabolism
MH  - Histocompatibility Antigens Class II/*genetics
MH  - Humans
MH  - Immunologic Deficiency Syndromes/*genetics
MH  - Mutation
MH  - *Promoter Regions, Genetic
MH  - RNA/analysis
PMC - PMC362172
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
PMCR- 1989/01/01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
PHST- 1989/01/01 00:00 [pmc-release]
AID - 10.1128/mcb.9.1.296-302.1989 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1989 Jan;9(1):296-302. doi: 10.1128/mcb.9.1.296-302.1989.