PMID- 24672780
OWN - NLM
STAT- MEDLINE
DCOM- 20150513
LR  - 20211203
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2014
DP  - 2014
TI  - Mesenchymal stem cells expressing brain-derived neurotrophic factor enhance 
      endogenous neurogenesis in an ischemic stroke model.
PG  - 129145
LID - 10.1155/2014/129145 [doi]
LID - 129145
AB  - Numerous studies have reported that mesenchymal stem cells (MSCs) can ameliorate 
      neurological deficits in ischemic stroke models. Among the various hypotheses 
      that have been suggested to explain the therapeutic mechanism underlying these 
      observations, neurogenesis is thought to be critical. To enhance the therapeutic 
      benefits of human bone marrow-derived MSCs (hBM-MSCs), we efficiently modified 
      hBM-MSCs by introduction of the brain-derived neurotrophic factor (BDNF) gene via 
      adenoviral transduction mediated by cell-permeable peptides and investigated 
      whether BDNF-modified hBM-MSCs (MSCs-BDNF) contributed to functional recovery and 
      endogenous neurogenesis in a rat model of middle cerebral artery occlusion 
      (MCAO). Transplantation of MSCs induced the proliferation of 
      5-bromo-2'-deoxyuridine (BrdU-) positive cells in the subventricular zone. 
      Transplantation of MSCs-BDNF enhanced the proliferation of endogenous neural stem 
      cells more significantly, while suppressing cell death. Newborn cells 
      differentiated into doublecortin (DCX-) positive neuroblasts and Neuronal Nuclei 
      (NeuN-) positive mature neurons in the subventricular zone and ischemic boundary 
      at higher rates in animals with MSCs-BDNF compared with treatment using solely 
      phosphate buffered saline (PBS) or MSCs. Triphenyltetrazolium chloride staining 
      and behavioral analysis revealed greater functional recovery in animals with 
      MSCs-BDNF compared with the other groups. MSCs-BDNF exhibited effective 
      therapeutic potential by protecting cell from apoptotic death and enhancing 
      endogenous neurogenesis.
FAU - Jeong, Chang Hyun
AU  - Jeong CH
AD  - Department of Biomedical Science, College of Medicine, The Catholic University of 
      Korea, Seoul 137-701, Republic of Korea.
FAU - Kim, Seong Muk
AU  - Kim SM
AD  - Postech-Catholic Biomedical Engineering Institute, The Catholic University of 
      Korea, Seoul 137-701, Republic of Korea.
FAU - Lim, Jung Yeon
AU  - Lim JY
AD  - Department of Biomedical Science, College of Medicine, The Catholic University of 
      Korea, Seoul 137-701, Republic of Korea.
FAU - Ryu, Chung Heon
AU  - Ryu CH
AD  - Postech-Catholic Biomedical Engineering Institute, The Catholic University of 
      Korea, Seoul 137-701, Republic of Korea.
FAU - Jun, Jin Ae
AU  - Jun JA
AD  - Department of Biomedical Science, College of Medicine, The Catholic University of 
      Korea, Seoul 137-701, Republic of Korea.
FAU - Jeun, Sin-Soo
AU  - Jeun SS
AD  - Department of Biomedical Science, College of Medicine, The Catholic University of 
      Korea, Seoul 137-701, Republic of Korea ; Department of Neurosurgery, Seoul St. 
      Mary's Hospital, The Catholic University of Korea, Seoul 137-701, Republic of 
      Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140205
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Protein)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Apoptosis
MH  - Brain Ischemia/complications/physiopathology/*therapy
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Doublecortin Protein
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Infarction, Middle Cerebral Artery/complications/physiopathology/therapy
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Middle Aged
MH  - *Neurogenesis
MH  - Rats, Sprague-Dawley
MH  - Stroke/complications/physiopathology/*therapy
MH  - Young Adult
PMC - PMC3933216
EDAT- 2014/03/29 06:00
MHDA- 2015/05/15 06:00
PMCR- 2014/02/05
CRDT- 2014/03/28 06:00
PHST- 2013/09/24 00:00 [received]
PHST- 2013/12/29 00:00 [accepted]
PHST- 2014/03/28 06:00 [entrez]
PHST- 2014/03/29 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
PHST- 2014/02/05 00:00 [pmc-release]
AID - 10.1155/2014/129145 [doi]
PST - ppublish
SO  - Biomed Res Int. 2014;2014:129145. doi: 10.1155/2014/129145. Epub 2014 Feb 5.