PMID- 24673554
OWN - NLM
STAT- MEDLINE
DCOM- 20160504
LR  - 20140707
IS  - 1543-8392 (Electronic)
IS  - 1543-8384 (Linking)
VI  - 11
IP  - 7
DP  - 2014 Jul 7
TI  - Biomimetic microfluidic device for in vitro antihypertensive drug evaluation.
PG  - 2009-15
LID - 10.1021/mp5000532 [doi]
AB  - Microfluidic devices have emerged as revolutionary, novel platforms for in vitro 
      drug evaluation. In this work, we developed a facile method for evaluating 
      antihypertensive drugs using a microfluidic chip. This microfluidic chip was 
      generated using the elastic material poly(dimethylsiloxane) (PDMS) and a 
      microchannel structure that simulated a blood vessel as fabricated on the chip. 
      We then cultured human umbilical vein endothelial cells (HUVECs) inside the 
      channel. Different pressures and shear stresses could be applied on the cells. 
      The generated vessel mimics can be used for evaluating the safety and effects of 
      antihypertensive drugs. Here, we used hydralazine hydrochloride as a model drug. 
      The results indicated that hydralazine hydrochloride effectively decreased the 
      pressure-induced dysfunction of endothelial cells. This work demonstrates that 
      our microfluidic system provides a convenient and cost-effective platform for 
      studying cellular responses to drugs under mechanical pressure.
FAU - Li, Lei
AU  - Li L
AD  - Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, 
      Chinese Academy of Sciences & Beijing Key Laboratory of Cryo-Biomedical 
      Engineering , Beijing100190, China.
FAU - Lv, Xiaoqing
AU  - Lv X
FAU - Ostrovidov, Serge
AU  - Ostrovidov S
FAU - Shi, Xuetao
AU  - Shi X
FAU - Zhang, Ning
AU  - Zhang N
FAU - Liu, Jing
AU  - Liu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140407
PL  - United States
TA  - Mol Pharm
JT  - Molecular pharmaceutics
JID - 101197791
RN  - 0 (Antihypertensive Agents)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Nylons)
RN  - 0 (poly(dimethylsiloxane)-polyamide copolymer)
RN  - 26NAK24LS8 (Hydralazine)
SB  - IM
MH  - Antihypertensive Agents/chemistry/*pharmacology
MH  - Biomimetic Materials/chemistry/*pharmacology
MH  - Biomimetics/*methods
MH  - Blood Vessels/drug effects
MH  - Cells, Cultured
MH  - Dimethylpolysiloxanes/chemistry
MH  - Drug Evaluation/methods
MH  - Endothelial Cells/drug effects
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Hydralazine/chemistry/pharmacology
MH  - In Vitro Techniques/methods
MH  - Lab-On-A-Chip Devices/*methods
MH  - Microfluidics/methods
MH  - Nylons/chemistry
MH  - Pressure
MH  - Shear Strength/drug effects
MH  - Stress, Mechanical
EDAT- 2014/03/29 06:00
MHDA- 2016/05/05 06:00
CRDT- 2014/03/29 06:00
PHST- 2014/03/29 06:00 [entrez]
PHST- 2014/03/29 06:00 [pubmed]
PHST- 2016/05/05 06:00 [medline]
AID - 10.1021/mp5000532 [doi]
PST - ppublish
SO  - Mol Pharm. 2014 Jul 7;11(7):2009-15. doi: 10.1021/mp5000532. Epub 2014 Apr 7.