PMID- 24675277
OWN - NLM
STAT- MEDLINE
DCOM- 20141215
LR  - 20220316
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 117
DP  - 2014 May 1
TI  - An in vitro study on the interaction of hydroxyapatite nanoparticles and bone 
      marrow mesenchymal stem cells for assessing the toxicological behaviour.
PG  - 389-97
LID - S0927-7765(14)00060-5 [pii]
LID - 10.1016/j.colsurfb.2014.02.004 [doi]
AB  - Mesenchymal stem cells or multipotent progenitor cells isolated from bone marrow 
      presents close resemblance to the natural in vivo milieu and hence preferred more 
      than the conventional cell culture systems to predict the toxicological behavior 
      of bio-nano interaction. The objective of the present study is to evaluate the 
      molecular toxicity of hydroxyapatite nanoparticles (HANPs) using mouse bone 
      marrow mesenchymal stem cells (BMSCs). In-house synthesized HANPs (50 nm) were 
      used to study the cytotoxicity, nano particle uptake, effect on cyto skeletal 
      arrangement, oxidative stress response and apoptotic behavior with the confluent 
      BMSCs as per standard protocols. The results of the MTT assay indicated that 
      HANPs does not induce cytotoxicity up to 800 mug/mL. It was also observed that 
      oxidative stress related apoptosis and reactive oxygen species (ROS) production 
      following nanoparticle treatment was similar to that of control (cells without 
      treatment). Hence it can be concluded that the in-house synthesized HANPs are 
      non-toxic/safe at the molecular level suggesting that the HANPs are compatible to 
      BMSCs. Further, the in vitro BMSCs cell culture can be used as a model for 
      evaluating the preliminary toxicity of nanomaterials.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Remya, N S
AU  - Remya NS
AD  - Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences 
      and Technology, Poojapura, Thiruvananthapuram 695012, Kerala, India.
FAU - Syama, S
AU  - Syama S
AD  - Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences 
      and Technology, Poojapura, Thiruvananthapuram 695012, Kerala, India.
FAU - Gayathri, V
AU  - Gayathri V
AD  - Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences 
      and Technology, Poojapura, Thiruvananthapuram 695012, Kerala, India.
FAU - Varma, H K
AU  - Varma HK
AD  - Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences 
      and Technology, Poojapura, Thiruvananthapuram 695012, Kerala, India.
FAU - Mohanan, P V
AU  - Mohanan PV
AD  - Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences 
      and Technology, Poojapura, Thiruvananthapuram 695012, Kerala, India. Electronic 
      address: mohanpv@sctimst.ac.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140228
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Actins)
RN  - 91D9GV0Z28 (Durapatite)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 7)
SB  - IM
EIN - Colloids Surf B Biointerfaces. 2021 Nov;207:112000. PMID: 34332305
MH  - Actins/metabolism
MH  - Animals
MH  - Bone Marrow Cells/*cytology/drug effects/enzymology
MH  - Caspase 3/metabolism
MH  - Caspase 7/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cell Separation
MH  - Cell Shape/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cytoskeleton/drug effects/metabolism
MH  - Durapatite/chemical synthesis/*toxicity
MH  - Mesenchymal Stem Cells/*cytology/drug effects/enzymology
MH  - Mice
MH  - Nanoparticles/*toxicity/ultrastructure
MH  - Particle Size
MH  - *Toxicity Tests
OTO - NOTNLM
OT  - Hydroxyapatite
OT  - Nanoparticles
OT  - Stem cells
EDAT- 2014/03/29 06:00
MHDA- 2014/12/17 06:00
CRDT- 2014/03/29 06:00
PHST- 2013/12/02 00:00 [received]
PHST- 2014/01/02 00:00 [revised]
PHST- 2014/02/03 00:00 [accepted]
PHST- 2014/03/29 06:00 [entrez]
PHST- 2014/03/29 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - S0927-7765(14)00060-5 [pii]
AID - 10.1016/j.colsurfb.2014.02.004 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2014 May 1;117:389-97. doi: 
      10.1016/j.colsurfb.2014.02.004. Epub 2014 Feb 28.