PMID- 24676340
OWN - NLM
STAT- MEDLINE
DCOM- 20141218
LR  - 20211021
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 33
IP  - 6
DP  - 2014 Jun
TI  - TNF-alpha promotes gallbladder cancer cell growth and invasion through autocrine 
      mechanisms.
PG  - 1431-40
LID - 10.3892/ijmm.2014.1711 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been suggested to be a putative tumor 
      promoter gene, and autocrine of TNF-alpha expression has been found in colon cancer 
      and ovarian cancer. As the role of autocrine TNF-alpha in human gallbladder cancer 
      has not yet been elucidated, the present study examined the expression of TNF-alpha 
      in gallbladder cancer-derived cell lines. Based on the data, TNF-alpha mRNA and TNF-alpha 
      protein expression differed significantly different between the cell lines. In 
      addition, using siRNA targeting TNF-alpha, the vector, pGPU-GFP-siTNF-alpha, was 
      constructed and then transfected into the SGC-996 cells (gallbladder cancer cell 
      line) which express high levels of endogenous TNF-alpha. In vitro experiments 
      indicated that the silencing of TNF-alpha in the SGC-996 cells significantly 
      suppressed proliferation and invasion. However, apoptosis was not induced by the 
      silencing of TNF-alpha. Furthermore, we traced the mechanisms underlying these 
      effects and found that the silencing of TNF-alpha affected the TNF-alpha-AKT-NF-kappaB-Bcl-2 
      pathway in the SGC-996 cells. Our data provide evidence that autocrine TNF-alpha 
      plays a role as a tumor promoter gene in gallbladder cancer cells, possibly by 
      promoting proliferation and invasion through autocrine mechanisms.
FAU - Zhu, Guangwei
AU  - Zhu G
AD  - Department of Hepatobiliary Surgery, The Affiliated Union Hospital of Fujian 
      Medical University, Fuzhou, Fujian, P.R. China.
FAU - Du, Qiang
AU  - Du Q
AD  - Department of Hepatobiliary Surgery, The Affiliated Union Hospital of Fujian 
      Medical University, Fuzhou, Fujian, P.R. China.
FAU - Wang, Xiaoqian
AU  - Wang X
AD  - Department of Hepatobiliary Surgery, The Affiliated Union Hospital of Fujian 
      Medical University, Fuzhou, Fujian, P.R. China.
FAU - Tang, Nanhong
AU  - Tang N
AD  - Department of Hepatobiliary Surgery, The Affiliated Union Hospital of Fujian 
      Medical University, Fuzhou, Fujian, P.R. China.
FAU - She, Feifei
AU  - She F
AD  - Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of 
      Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, P.R. China.
FAU - Chen, Yanling
AU  - Chen Y
AD  - Department of Hepatobiliary Surgery, The Affiliated Union Hospital of Fujian 
      Medical University, Fuzhou, Fujian, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140324
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Apoptosis/genetics/physiology
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics/physiology
MH  - Cell Proliferation/physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Gallbladder Neoplasms/etiology/genetics/*metabolism
MH  - Humans
MH  - Receptors, Tumor Necrosis Factor, Type I/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
PMC - PMC4055436
EDAT- 2014/03/29 06:00
MHDA- 2014/12/19 06:00
PMCR- 2014/03/24
CRDT- 2014/03/29 06:00
PHST- 2013/11/29 00:00 [received]
PHST- 2014/03/11 00:00 [accepted]
PHST- 2014/03/29 06:00 [entrez]
PHST- 2014/03/29 06:00 [pubmed]
PHST- 2014/12/19 06:00 [medline]
PHST- 2014/03/24 00:00 [pmc-release]
AID - ijmm-33-06-1431 [pii]
AID - 10.3892/ijmm.2014.1711 [doi]
PST - ppublish
SO  - Int J Mol Med. 2014 Jun;33(6):1431-40. doi: 10.3892/ijmm.2014.1711. Epub 2014 Mar 
      24.