PMID- 24678026
OWN - NLM
STAT- MEDLINE
DCOM- 20150611
LR  - 20200930
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 102
IP  - 8
DP  - 2014 Nov
TI  - Biomineralization regulation by nano-sized features in silk fibroin proteins: 
      synthesis of water-dispersible nano-hydroxyapatite.
PG  - 1720-9
LID - 10.1002/jbm.b.33157 [doi]
AB  - In the present study, silk fibroin (SF) was used as a template to prepare 
      nano-hydroxyapatite (nano-HA) via a biomineralization process. We observed that 
      the content of SF affected both the morphology and water dispersibility of 
      nano-HA particles. Scanning electron microscope (SEM), transmission electron 
      microscopy (TEM), X-ray diffraction (XRD), zetasizer, and Fourier transform 
      infrared spectroscopy (FTIR) were used to examine nano-HA particle features 
      including the surface morphology, aggregation performance, and crystallization. 
      Rod-like nano-HA particles with desired water dispersibility were achieved when 
      the ratio of SF/HA (calculated) was above 7:3. SEM, TEM, and zeta potential 
      results revealed that nano-HA particles were enclosed by the SF which formed a 
      negative charge layer preventing the aggregation of HA nanoparticles in aqueous 
      solution. Moreover, the nano-HA particles were able to re-disperse in water 
      without precipitation for two weeks at room temperature, 60 degrees C, and 90 degrees C. Our work 
      suggested a facile and effective approach of designing water-dispersible nano-HA 
      particles which may have wide potential application in tissue engineering 
      especially bone regeneration.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Huang, Xiaowei
AU  - Huang X
AD  - National Engineering Laboratory for Modern Silk, College of Textile and Clothing 
      Engineering, Soochow University, Suzhou, 215123, People's Republic of China.
FAU - Liu, Xi
AU  - Liu X
FAU - Liu, Shanshan
AU  - Liu S
FAU - Zhang, Aili
AU  - Zhang A
FAU - Lu, Qiang
AU  - Lu Q
FAU - Kaplan, David L
AU  - Kaplan DL
FAU - Zhu, Hesun
AU  - Zhu H
LA  - eng
GR  - EB002520/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140328
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (fibroin, silkworm)
RN  - 059QF0KO0R (Water)
RN  - 9007-76-5 (Fibroins)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Animals
MH  - Bombyx
MH  - Bone Regeneration
MH  - *Calcification, Physiologic
MH  - Durapatite/*chemistry
MH  - Fibroins/*chemistry
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Particle Size
MH  - Tissue Engineering/methods
MH  - Water/*chemistry
OTO - NOTNLM
OT  - biomineralization
OT  - hydroxyapatite
OT  - silk
OT  - water-dispersible
EDAT- 2014/03/29 06:00
MHDA- 2015/06/13 06:00
CRDT- 2014/03/29 06:00
PHST- 2013/12/26 00:00 [received]
PHST- 2014/02/28 00:00 [revised]
PHST- 2014/03/13 00:00 [accepted]
PHST- 2014/03/29 06:00 [entrez]
PHST- 2014/03/29 06:00 [pubmed]
PHST- 2015/06/13 06:00 [medline]
AID - 10.1002/jbm.b.33157 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2014 Nov;102(8):1720-9. doi: 
      10.1002/jbm.b.33157. Epub 2014 Mar 28.