PMID- 24680746
OWN - NLM
STAT- MEDLINE
DCOM- 20141208
LR  - 20220311
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1563
DP  - 2014 May 14
TI  - Improvement of human umbilical cord mesenchymal stem cell transplantation on 
      glial cell and behavioral function in a neonatal model of periventricular white 
      matter damage.
PG  - 13-21
LID - S0006-8993(14)00392-8 [pii]
LID - 10.1016/j.brainres.2014.03.030 [doi]
AB  - Periventricular white matter damage (PWMD) also termed periventricular 
      leucomalacia in the preterm infant is of particular importance because no 
      targeted therapy is presently available. Human umbilical cord mesenchymal stem 
      cells (hUC-MSCs) have been studied in a variety of adult brain injury-related 
      neurological disorders. Our aim was to determine whether hUC-MSC transplantation 
      improves glial cell function in cerebral white matter and long-term behavioral 
      function in a PWMD rat model. Rats on postnatal day (P) 3 underwent a permanent 
      ligation of the left common carotid artery followed by 6% O2 for 4h. Immediately 
      after the hypoxic-ischemic (HI), rats received a single intraperitoneal injection 
      of hUC-MSCs, which were co-cultured with 5-bromodeoxyuridine (BrdU). BrdU(+) 
      cells in the brain were tested 24h after transplantation. Second, rats received 
      hUC-MSC treatment once a day for 3 consecutive days. Glial cells 
      (oligodendrocytes, astrocytes and microglia) were examined on 7 and 18 days 
      post-HI, and behavioral outcomes were tested 27 days post-HI. Significantly, 
      hUC-MSCs migrated mainly into the injured hemisphere. In addition, hUC-MSC 
      treatment improved the long-term functional outcomes of rats, increased mature 
      oligodendrocyte counts, and decreased the number of reactive astrocytes and 
      activated microglia quantities after HI-induced damage in the premature brain. 
      These results suggest that hUC-MSCs can pass through the blood-brain barrier and 
      migrate towards the lesion site to improve brain damage. Therefore, hUC-MSCs have 
      the potential to be utilized as a novel therapeutic strategy for PWMD.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Zhu, Li-Hua
AU  - Zhu LH
AD  - Research Center for Learning Science, Southeast University, 2 Sipai Lou, Xuanwu 
      District, Nanjing 210096, China.
FAU - Bai, Xiang
AU  - Bai X
AD  - Teaching and Research Section of Pediatrics, Clinical Medical College, Southeast 
      University, 87 Dingjia Qiao, Gulou District, Nanjing 210009, China.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Teaching and Research Section of Pediatrics, Clinical Medical College, Southeast 
      University, 87 Dingjia Qiao, Gulou District, Nanjing 210009, China.
FAU - Wang, Shi-Yu
AU  - Wang SY
AD  - Teaching and Research Section of Pediatrics, Clinical Medical College, Southeast 
      University, 87 Dingjia Qiao, Gulou District, Nanjing 210009, China.
FAU - Li, Wei
AU  - Li W
AD  - Stem Cell Engineering Research Center, 1 Binhe Road, Gaogang District, Taizhou 
      225300, China.
FAU - Jiang, Li
AU  - Jiang L
AD  - Department of Pediatrics, Zhongda Hospital, Southeast University, 87 Dingjia 
      Qiao, Gulou District, Nanjing 210009, China. Electronic address: 
      jiangli77777@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140326
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Behavior, Animal
MH  - *Cord Blood Stem Cell Transplantation
MH  - Disease Models, Animal
MH  - Humans
MH  - Leukomalacia, Periventricular/*therapy
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Neuroglia/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Glial cell
OT  - Hypoxia-ischemia
OT  - Periventricular white matter damage
OT  - Umbilical cord mesenchymal stem cell
EDAT- 2014/04/01 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/04/01 06:00
PHST- 2013/11/12 00:00 [received]
PHST- 2014/03/06 00:00 [revised]
PHST- 2014/03/19 00:00 [accepted]
PHST- 2014/04/01 06:00 [entrez]
PHST- 2014/04/01 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0006-8993(14)00392-8 [pii]
AID - 10.1016/j.brainres.2014.03.030 [doi]
PST - ppublish
SO  - Brain Res. 2014 May 14;1563:13-21. doi: 10.1016/j.brainres.2014.03.030. Epub 2014 
      Mar 26.