PMID- 24680825
OWN - NLM
STAT- MEDLINE
DCOM- 20140623
LR  - 20181202
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 447
IP  - 1
DP  - 2014 Apr 25
TI  - Cell-to-cell propagation of intracellular signals fluorescently visualized with 
      acridine orange in the gastric glands of guinea pigs.
PG  - 38-43
LID - S0006-291X(14)00544-0 [pii]
LID - 10.1016/j.bbrc.2014.03.095 [doi]
AB  - Secretion from the gastric gland involves the activation of various types of 
      cells in a coordinated manner. In order to elucidate the mechanisms underlying 
      the coordination of secretion, we studied live fluorescence images of guinea pig 
      gastric glands stained with acridine orange (AO). On 2 muM AO staining, individual 
      cells were characterized by metachromatic colors and various intensities of 
      fluorescence. When the gland was stimulated with 100 muM of histamine, green 
      fluorescence was transiently increased in parietal cells and intermediate cells 
      and propagated along the gland for a long distance over many cells. Local 
      stimulation in a couple of cells with histamine in the presence of suramin also 
      induced propagation. However, the fluorescence response was suppressed by the 
      addition of H-89, a protein kinase A inhibitor. These findings suggest that a 
      cAMP-dependent signal propagates intercellularly through a variety of cells to 
      induce coordinated secretion in the entire gastric gland.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Fukushi, Yasuko
AU  - Fukushi Y
AD  - Medical Photonics Research Center, Hamamatsu University School of Medicine, 
      Hamamatsu, Japan.
FAU - Sakurai, Takashi
AU  - Sakurai T
AD  - Medical Photonics Research Center, Hamamatsu University School of Medicine, 
      Hamamatsu, Japan.
FAU - Terakawa, Susumu
AU  - Terakawa S
AD  - Medical Photonics Research Center, Hamamatsu University School of Medicine, 
      Hamamatsu, Japan. Electronic address: terakawa@hama-med.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20140327
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Isoquinolines)
RN  - 0 (Sulfonamides)
RN  - 820484N8I3 (Histamine)
RN  - F30N4O6XVV (Acridine Orange)
RN  - M876330O56 (N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Calcium/physiology
MH  - Cell Communication/drug effects/*physiology
MH  - Gastric Mucosa/*physiology
MH  - Guinea Pigs
MH  - Histamine/pharmacology
MH  - Isoquinolines/pharmacology
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Parietal Cells, Gastric/drug effects/metabolism/*physiology
MH  - *Signal Transduction
MH  - Sulfonamides/pharmacology
OTO - NOTNLM
OT  - A-kinase system
OT  - Fluorescence imaging
OT  - Gap junction
OT  - Parietal cell
EDAT- 2014/04/01 06:00
MHDA- 2014/06/24 06:00
CRDT- 2014/04/01 06:00
PHST- 2014/03/14 00:00 [received]
PHST- 2014/03/19 00:00 [accepted]
PHST- 2014/04/01 06:00 [entrez]
PHST- 2014/04/01 06:00 [pubmed]
PHST- 2014/06/24 06:00 [medline]
AID - S0006-291X(14)00544-0 [pii]
AID - 10.1016/j.bbrc.2014.03.095 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2014 Apr 25;447(1):38-43. doi: 
      10.1016/j.bbrc.2014.03.095. Epub 2014 Mar 27.