PMID- 24682997
OWN - NLM
STAT- MEDLINE
DCOM- 20141117
LR  - 20211021
IS  - 1976-3794 (Electronic)
IS  - 1225-8873 (Print)
IS  - 1225-8873 (Linking)
VI  - 52
IP  - 4
DP  - 2014 Apr
TI  - Development of a chimeric strain of porcine reproductive and respiratory syndrome 
      virus with an infectious clone and a Korean dominant field strain.
PG  - 345-9
LID - 10.1007/s12275-014-4074-4 [doi]
AB  - The K418 chimeric virus of porcine reproductive and respiratory syndrome virus 
      (PRRSV) was engineered by replacing the genomic region containing structure 
      protein genes of an infectious clone of PRRSV, FL12, with the same region 
      obtained from a Korean dominant field strain, LMY. The K418 reached 10(6) 
      TCID50/ml of viral titer with similar growth kinetics to those of parental 
      strains and had a cross-reactive neutralizing antibody response to field serum 
      from the entire country. The chimeric clone pK418 can be used as a practical tool 
      for further studying the molecular characteristics of PRRSV proteins through 
      genetic manipulation. Furthermore, successful construction of the K418 will allow 
      for the development of customized vaccine candidates against PRRSV, which has 
      evolved rapidly in Korea.
FAU - Lee, Jung-Ah
AU  - Lee JA
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 143-701, Republic of Korea.
FAU - Lee, Nak-Hyung
AU  - Lee NH
FAU - Lee, Sang-Won
AU  - Lee SW
FAU - Park, Seung-Yong
AU  - Park SY
FAU - Song, Chang-Seon
AU  - Song CS
FAU - Choi, In-Soo
AU  - Choi IS
FAU - Lee, Joong-Bok
AU  - Lee JB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140329
PL  - Korea (South)
TA  - J Microbiol
JT  - Journal of microbiology (Seoul, Korea)
JID - 9703165
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Korea
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*growth & 
      development/immunology/isolation & purification
MH  - *Recombination, Genetic
MH  - Swine
MH  - Viral Load
PMC - PMC7091204
EDAT- 2014/04/01 06:00
MHDA- 2014/11/18 06:00
PMCR- 2020/03/24
CRDT- 2014/04/01 06:00
PHST- 2014/02/05 00:00 [received]
PHST- 2014/03/13 00:00 [accepted]
PHST- 2014/03/11 00:00 [revised]
PHST- 2014/04/01 06:00 [entrez]
PHST- 2014/04/01 06:00 [pubmed]
PHST- 2014/11/18 06:00 [medline]
PHST- 2020/03/24 00:00 [pmc-release]
AID - 4074 [pii]
AID - 10.1007/s12275-014-4074-4 [doi]
PST - ppublish
SO  - J Microbiol. 2014 Apr;52(4):345-9. doi: 10.1007/s12275-014-4074-4. Epub 2014 Mar 
      29.