PMID- 24685168
OWN - NLM
STAT- MEDLINE
DCOM- 20140816
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1340
DP  - 2014 May 2
TI  - Rapid and high-throughput determination of endogenous cytokinins in Oryza sativa 
      by bare Fe3O4 nanoparticles-based magnetic solid-phase extraction.
PG  - 146-50
LID - S0021-9673(14)00425-7 [pii]
LID - 10.1016/j.chroma.2014.03.030 [doi]
AB  - A rapid method was developed for determination of endogenous cytokinins (CKs) 
      based on magnetic solid-phase extraction (MSPE) followed by ultra-performance 
      liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). We illustrated the 
      hydrophilic character of bare Fe3O4 nanoparticles that were directly used as a 
      MSPE sorbent for rapid enrichment of endogenous CKs from complex plant extract. 
      To the best of our knowledge, this is the first report of bare Fe3O4 directly 
      used as efficient extraction sorbent to enrich target CKs based on hydrophilic 
      interaction. Under the optimized conditions, a rapid, sensitive and 
      high-throughput method for the determination of 16 CKs was established by 
      combination of MSPE with UPLC-MS/MS. Good linearity was obtained with correlation 
      coefficients (r) from 0.9902 to 0.9998. The limits of detection (LODs) and 
      quantification (LOQs) ranged from 1.2 pg mL(-1) to 391.3 pg mL(-1) and 4.1 pg 
      mL(-1) to 1304.3 pg mL(-1), respectively. 16 CKs could be successfully determined 
      in spiked sample with 80.6-117.3% recoveries and the relative standard deviations 
      (RSDs) were less than 16.6%. Finally, 10 endogenous CKs were successfully 
      quantified in 50mg Oryza sativa sample using the developed MSPE-UPLC-MS/MS 
      method.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Cai, Bao-Dong
AU  - Cai BD
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Zhu, Jiu-Xia
AU  - Zhu JX
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Gao, Qiang
AU  - Gao Q
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China; 
      Engineering Research Center of Nano-Geomaterials of Ministry of Education, 
      Department of Chemistry, China University of Geosciences, Wuhan 430074, China.
FAU - Luo, Dan
AU  - Luo D
AD  - Shimadzu Global COE for Application & Technical Development, Shimadzu 
      International Trading (Shanghai) Co., Ltd, Shanghai 200052, China.
FAU - Yuan, Bi-Feng
AU  - Yuan BF
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China. 
      Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140318
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Cytokinins)
RN  - 0 (Ferric Compounds)
RN  - 0 (Magnetite Nanoparticles)
RN  - 1K09F3G675 (ferric oxide)
SB  - IM
MH  - Chromatography, Liquid/methods
MH  - Cytokinins/*analysis/chemistry/isolation & purification
MH  - Ferric Compounds/*chemistry
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Limit of Detection
MH  - Magnetite Nanoparticles/*chemistry
MH  - Oryza/*chemistry
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - Cytokinins
OT  - Fe(3)O(4) nanoparticles
OT  - Hydrophilic interaction
OT  - Magnetic solid-phase extraction
OT  - UPLC-MS/MS
EDAT- 2014/04/02 06:00
MHDA- 2014/08/17 06:00
CRDT- 2014/04/02 06:00
PHST- 2013/11/14 00:00 [received]
PHST- 2014/03/10 00:00 [revised]
PHST- 2014/03/11 00:00 [accepted]
PHST- 2014/04/02 06:00 [entrez]
PHST- 2014/04/02 06:00 [pubmed]
PHST- 2014/08/17 06:00 [medline]
AID - S0021-9673(14)00425-7 [pii]
AID - 10.1016/j.chroma.2014.03.030 [doi]
PST - ppublish
SO  - J Chromatogr A. 2014 May 2;1340:146-50. doi: 10.1016/j.chroma.2014.03.030. Epub 
      2014 Mar 18.