PMID- 24689455
OWN - NLM
STAT- MEDLINE
DCOM- 20141104
LR  - 20211203
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 143
IP  - 2
DP  - 2014 Oct
TI  - Dendritic cells treated with crude Plasmodium berghei extracts acquire 
      immune-modulatory properties and suppress the development of autoimmune 
      neuroinflammation.
PG  - 164-73
LID - 10.1111/imm.12298 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells specifically 
      targeted during Plasmodium infection. Upon infection, DCs show impaired antigen 
      presentation and T-cell activation abilities. In this study, we aimed to evaluate 
      whether cellular extracts obtained from Plasmodium berghei-infected erythrocytes 
      (PbX) modulate DCs phenotypically and functionally and the potential therapeutic 
      usage of PbX-modulated DCs in the control of experimental autoimmune 
      encephalomyelitis (EAE, the mouse model for human multiple sclerosis). We found 
      that PbX-treated DCs have impaired maturation and stimulated the generation of 
      regulatory T cells when cultured with naive T lymphocytes in vitro. When 
      adoptively transferred to C57BL/6 mice the EAE severity was reduced. Disease 
      amelioration correlated with a diminished infiltration of cytokine-producing T 
      cells in the central nervous system as well as the suppression of 
      encephalitogenic T cells. Our study shows that extracts obtained from P. 
      berghei-infected erythrocytes modulate DCs towards an immunosuppressive 
      phenotype. In addition, the adoptive transfer of PbX-modulated DCs was able to 
      ameliorate EAE development through the suppression of specific cellular immune 
      responses towards neuro-antigens. To our knowledge, this is the first study to 
      present evidence that DCs treated with P. berghei extracts are able to control 
      autoimmune neuroinflammation.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Thome, Rodolfo
AU  - Thome R
AD  - Department of Structural and Functional Biology, Institute of Biology, University 
      of Campinas, Campinas, Sao Paulo, Brazil.
FAU - Issayama, Luidy K
AU  - Issayama LK
FAU - Alves da Costa, Thiago
AU  - Alves da Costa T
FAU - Gangi, Rosaria D
AU  - Gangi RD
FAU - Ferreira, Isadora T
AU  - Ferreira IT
FAU - Raposo, Catarina
AU  - Raposo C
FAU - Lopes, Stefanie C P
AU  - Lopes SC
FAU - da Cruz Hofling, Maria Alice
AU  - da Cruz Hofling MA
FAU - Costa, Fabio T M
AU  - Costa FT
FAU - Verinaud, Liana
AU  - Verinaud L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Cytokines)
SB  - IM
MH  - *Adoptive Transfer
MH  - Animals
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Dendritic Cells/immunology/metabolism/parasitology/*transplantation
MH  - Female
MH  - Immunity, Cellular
MH  - Immunosuppression Therapy/*methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neuritis, Autoimmune, Experimental/immunology/metabolism/parasitology/*prevention 
      & control
MH  - Phenotype
MH  - Plasmodium berghei/*immunology
MH  - T-Lymphocytes, Regulatory/*immunology/metabolism/parasitology
MH  - Time Factors
PMC - PMC4172133
OTO - NOTNLM
OT  - Plasmodium extracts
OT  - dendritic cells
OT  - experimental autoimmune encephalomyelitis
OT  - neuroinflammation
EDAT- 2014/04/03 06:00
MHDA- 2014/11/05 06:00
PMCR- 2015/10/01
CRDT- 2014/04/03 06:00
PHST- 2013/12/17 00:00 [received]
PHST- 2014/03/27 00:00 [revised]
PHST- 2014/03/28 00:00 [accepted]
PHST- 2014/04/03 06:00 [entrez]
PHST- 2014/04/03 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
PHST- 2015/10/01 00:00 [pmc-release]
AID - 10.1111/imm.12298 [doi]
PST - ppublish
SO  - Immunology. 2014 Oct;143(2):164-73. doi: 10.1111/imm.12298.