PMID- 24690077
OWN - NLM
STAT- MEDLINE
DCOM- 20160126
LR  - 20150106
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 50
IP  - 1
DP  - 2015 Feb
TI  - Evaluation of gingival crevicular fluid levels of tissue plasminogen activator, 
      plasminogen activator inhibitor 2, matrix metalloproteinase-3 and interleukin 1-beta 
      in patients with different periodontal diseases.
PG  - 44-51
LID - 10.1111/jre.12179 [doi]
AB  - OBJECTIVES: The purpose of this study was to evaluate the gingival crevicular 
      fluid levels of interleukin-1beta (IL-1beta), matrix metalloproteinases-3 (MMP-3), 
      tissue type plasminogen activator (t-PA) and plasminogen activator inhibitor 2 
      (PAI-2) in patients with chronic periodontitis, aggressive periodontitis (AgP) 
      and healthy individuals (controls). MATERIAL AND METHODS: Systemically healthy 
      (21 chronic periodontitis, 23 AgP and 20 controls) subjects were included in this 
      study. Plaque index, gingival index, probing pocket depth and clinical attachment 
      level were recorded and gingival crevicular fluid samples were collected. Assays 
      for IL-1beta, MMP-3, t-PA and PAI-2 levels in gingival crevicular fluid were carried 
      out by an enzyme-linked immunosorbent assay. The one-sample Kolmogorov-Smirnov 
      test, Mann-Whitney U test and Spearman correlation coefficient were used for data 
      analyses. RESULTS: Gingival crevicular fluid levels of t-PA and IL-1beta were 
      significantly higher in chronic periodontitis and AgP groups than in the control 
      group (p < 0.001). MMP-3 levels in gingival crevicular fluid were detected as 
      significantly higher in the chronic periodontitis and AgP groups compared with 
      the control group (p < 0.05). The t-PA/PAI-2 rate of patients with chronic 
      periodontitis and AgP were significantly higher than the control group 
      (p < 0.05). The positive correlations were found among the PAI-2, t-PA, IL-1beta and 
      MMP-3 levels in gingival crevicular fluid. The volume of the gingival crevicular 
      fluid correlated with all of the clinical parameters (p < 0.001). There were 
      positive correlations between the gingival crevicular fluid levels of PAI-2 and 
      the probing pocket depth and between gingival crevicular fluid levels of PAI-2 
      and the clinical attachment level (p < 0.01). Similarly, significant correlations 
      were found between t-PA levels and probing pocket depth and between t-PA levels 
      and clinical attachment level measurements (p < 0.001). CONCLUSION: The present 
      data showed that gingival crevicular fluid levels of IL-1 beta, MMP-3 and t-PA 
      increased in periodontal disease regardless of the periodontitis type and played 
      a part in tissue destruction.
CI  - (c) 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Toyman, U
AU  - Toyman U
AD  - Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, 
      Turkey; The Health Ministry, Center of Oral and Dental Health, Bursa, Turkey.
FAU - Tuter, G
AU  - Tuter G
FAU - Kurtis, B
AU  - Kurtis B
FAU - Kivrak, E
AU  - Kivrak E
FAU - Bozkurt, S
AU  - Bozkurt S
FAU - Yucel, A A
AU  - Yucel AA
FAU - Serdar, M
AU  - Serdar M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140402
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Plasminogen Activator Inhibitor 2)
RN  - 0 (Serine Proteinase Inhibitors)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Aggressive Periodontitis/*metabolism
MH  - Chronic Periodontitis/*metabolism
MH  - Dental Plaque Index
MH  - Female
MH  - Fibrinolytic Agents/analysis
MH  - Gingival Crevicular Fluid/*chemistry
MH  - Humans
MH  - Interleukin-1beta/*analysis
MH  - Male
MH  - Matrix Metalloproteinase 3/*analysis
MH  - Middle Aged
MH  - Periodontal Attachment Loss/classification/metabolism
MH  - Periodontal Index
MH  - Periodontal Pocket/classification/metabolism
MH  - Plasminogen Activator Inhibitor 2/*analysis
MH  - Serine Proteinase Inhibitors/analysis
MH  - Tissue Plasminogen Activator/*analysis
MH  - Young Adult
OTO - NOTNLM
OT  - interleukin-1beta
OT  - matrix metalloproteinase-3
OT  - plasminogen activator inhibitor 2
OT  - tissue plasminogen activator
EDAT- 2014/04/03 06:00
MHDA- 2016/01/27 06:00
CRDT- 2014/04/03 06:00
PHST- 2014/02/19 00:00 [accepted]
PHST- 2014/04/03 06:00 [entrez]
PHST- 2014/04/03 06:00 [pubmed]
PHST- 2016/01/27 06:00 [medline]
AID - 10.1111/jre.12179 [doi]
PST - ppublish
SO  - J Periodontal Res. 2015 Feb;50(1):44-51. doi: 10.1111/jre.12179. Epub 2014 Apr 2.