PMID- 24690089
OWN - NLM
STAT- MEDLINE
DCOM- 20141201
LR  - 20240321
IS  - 1742-2094 (Electronic)
IS  - 1742-2094 (Linking)
VI  - 11
DP  - 2014 Apr 2
TI  - Co-grafting of neural stem cells with olfactory en sheathing cells promotes 
      neuronal restoration in traumatic brain injury with an anti-inflammatory 
      mechanism.
PG  - 66
LID - 10.1186/1742-2094-11-66 [doi]
AB  - BACKGROUND: We sought to investigate the effects of co-grafting neural stem cells 
      (NSCs) with olfactory ensheathing cells (OECs) on neurological behavior in rats 
      subjected to traumatic brain injury (TBI) and explore underlying molecular 
      mechanisms. METHODS: TBI was established by percussion device made through a 
      weight drop (50 g) from a 30 cm height. Cultured NSCs and OECs isolated from rats 
      were labeled by Hoechst 33342 (blue) and chloromethyl-benzamidodialkyl 
      carbocyanine (CM-Dil) (red), respectively. Then, NSCs and/or OECs, separately or 
      combined, were transplanted into the area surrounding the injury site. Fourteen 
      days after transplantation, neurological severity score (NSS) were recorded. The 
      brain tissue was harvested and processed for immunocytochemistry, terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and reverse 
      transcription-polymerase chain reaction (RT-PCR). RESULTS: Significant 
      neurological function improvement was observed in the three transplant groups, 
      compared to the TBI group, and co-transplantation gave rise to the best 
      improvement. Morphological evaluation showed that the number of neurons in cortex 
      from combination implantation was more than for other groups (P <0.05); 
      conversely, the number of apoptotic cells showed a significant decrease by TUNEL 
      staining. Transplanted NSCs and OECs could survive and migrate in the brain, and 
      the number of neurons differentiating from NSCs in the co-transplantation group 
      was significantly greater than in the NSCs group. At the molecular level, the 
      expressions of IL-6 and BAD in the co-graft group were found to be down regulated 
      significantly, when compared to either the NSC or OEC alone groups. CONCLUSION: 
      The present study demonstrates for the first time the optimal effects of 
      co-grafting NSCs and OECs as a new strategy for the treatment of TBI via an 
      anti-inflammation mechanism.
FAU - Liu, Su-Juan
AU  - Liu SJ
FAU - Zou, Yu
AU  - Zou Y
FAU - Belegu, Visar
AU  - Belegu V
FAU - Lv, Long-Yun
AU  - Lv LY
FAU - Lin, Na
AU  - Lin N
FAU - Wang, Ting-Yong
AU  - Wang TY
FAU - McDonald, John W
AU  - McDonald JW
FAU - Zhou, Xue
AU  - Zhou X
FAU - Xia, Qing-Jie
AU  - Xia QJ
AD  - Department of Histology, Embryology and Neurobiology, West China School of 
      Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, 
      China. 193374073@qq.com.
FAU - Wang, Ting-Hua
AU  - Wang TH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140402
PL  - England
TA  - J Neuroinflammation
JT  - Journal of neuroinflammation
JID - 101222974
RN  - 0 (3,3'-dioactadecyl-5,5'-di(4-sulfophenyl)oxacarbocyanine)
RN  - 0 (Benzimidazoles)
RN  - 0 (Carbocyanines)
RN  - 0 (Cytokines)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
RN  - P976261J69 (bisbenzimide ethoxide trihydrochloride)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Benzimidazoles
MH  - Brain Injuries/*therapy
MH  - Carbocyanines
MH  - Cell Differentiation
MH  - Cell Transplantation/*methods
MH  - Cells, Cultured
MH  - Cytokines/genetics/*metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Neural Stem Cells/physiology/*transplantation
MH  - Neurologic Examination
MH  - Olfactory Bulb/*cytology
MH  - Phosphopyruvate Hydratase/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Schwann Cells/physiology/*transplantation
PMC - PMC3977666
EDAT- 2014/04/03 06:00
MHDA- 2014/12/15 06:00
PMCR- 2014/04/02
CRDT- 2014/04/03 06:00
PHST- 2014/01/19 00:00 [received]
PHST- 2014/03/24 00:00 [accepted]
PHST- 2014/04/03 06:00 [entrez]
PHST- 2014/04/03 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PHST- 2014/04/02 00:00 [pmc-release]
AID - 1742-2094-11-66 [pii]
AID - 10.1186/1742-2094-11-66 [doi]
PST - epublish
SO  - J Neuroinflammation. 2014 Apr 2;11:66. doi: 10.1186/1742-2094-11-66.