PMID- 24690145
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140410
LR  - 20211021
IS  - 2045-824X (Print)
IS  - 2045-824X (Electronic)
IS  - 2045-824X (Linking)
VI  - 6
IP  - 1
DP  - 2014 Apr 1
TI  - Development of immortalized mouse aortic endothelial cell lines.
PG  - 7
LID - 10.1186/2045-824X-6-7 [doi]
AB  - BACKGROUND: The understanding of endothelial cell biology has been facilitated by 
      the availability of primary endothelial cell cultures from a variety of sites and 
      species; however, the isolation and maintenance of primary mouse aortic 
      endothelial cells (MAECs) remain a formidable challenge. Culturing MAECs is 
      difficult as they are prone to phenotypic drift during culture. Therefore, there 
      is a need to have a dependable in vitro culture system, wherein the primary 
      endothelial cells retain their properties and phenotypes. METHODS: Here, we 
      developed an effective method to prepare immortalized MAEC (iMAEC) lines. Primary 
      MAECs, initially isolated from aortic explants, were immortalized using a 
      retrovirus expressing polyoma middle T-antigen. Immortalized cells were then 
      incubated with DiI-acetylated-low density lipoprotein and sorted via flow 
      cytometry to isolate iMAECs. RESULTS: iMAECs expressed common markers of 
      endothelial cells, including PECAM1, eNOS, VE-cadherin, and von Willebrand 
      Factor. iMAECs aligned in the direction of imposed laminar shear and retained the 
      ability to form tubes. Using this method, we have generated iMAEC lines from 
      wild-type and various genetically modified mice such as p47phox-/-, eNOS-/-, and 
      caveolin-1-/-. CONCLUSION: In summary, generation of iMAEC lines from various 
      genetically modified mouse lines provides an invaluable tool to study vascular 
      biology and pathophysiology.
FAU - Ni, Chih-Wen
AU  - Ni CW
FAU - Kumar, Sandeep
AU  - Kumar S
FAU - Ankeny, Casey J
AU  - Ankeny CJ
FAU - Jo, Hanjoong
AU  - Jo H
AD  - Wallace H, Coulter Department of Biomedical Engineering Georgia Institute of 
      Technology and Emory University, 1760 Haygood Drive, Health Science Research 
      Building, E-170, Atlanta, GA 30322, USA. hanjoong.jo@bme.gatech.edu.
LA  - eng
GR  - P20 HL113451/HL/NHLBI NIH HHS/United States
GR  - R01 HL119798/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20140401
PL  - Romania
TA  - Vasc Cell
JT  - Vascular cell
JID - 101553155
PMC - PMC4230636
EDAT- 2014/04/03 06:00
MHDA- 2014/04/03 06:01
PMCR- 2014/04/01
CRDT- 2014/04/03 06:00
PHST- 2013/12/30 00:00 [received]
PHST- 2014/03/10 00:00 [accepted]
PHST- 2014/04/03 06:00 [entrez]
PHST- 2014/04/03 06:00 [pubmed]
PHST- 2014/04/03 06:01 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - 2045-824X-6-7 [pii]
AID - 10.1186/2045-824X-6-7 [doi]
PST - epublish
SO  - Vasc Cell. 2014 Apr 1;6(1):7. doi: 10.1186/2045-824X-6-7.