PMID- 24690609
OWN - NLM
STAT- MEDLINE
DCOM- 20160115
LR  - 20211021
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 15
IP  - 3
DP  - 2014
TI  - Detection of porcine reproductive and respiratory syndrome virus in oral fluid 
      from naturally infected pigs in a breeding herd.
PG  - 361-7
AB  - The objectives of the present study were to evaluate the anatomic localization of 
      porcine reproductive and respiratory syndrome virus (PRRSV) in naturally infected 
      pigs and to determine whether oral fluid could be used to detect the virus in 
      infected animals. Two sows, seven 2-month-old grower pigs, and 70 6-month-old 
      gilts were included in this study. PRRSV in sera and oral fluid were identified 
      by nested reverse transcription PCR (nRT-PCR) while lung, tonsil, and tissue 
      associated with oral cavity were subjected to nRT-PCR, immunohistochemistry, and 
      in situ hybridization. In sows, PRRSV was identified in oral fluid and tonsils. 
      PRRSV was also detected in oral fluid, tonsils, salivary glands, oral mucosa, and 
      lungs of all seven grower pigs. However, viremia was observed in only two grower 
      pigs. Double staining revealed that PRRSV was distributed in macrophages within 
      and adjacent to the tonsillar crypt epithelium. In gilts, the North American type 
      PRRSV field strain was detected 3 to 8 weeks after introducing these animals onto 
      the farm. These results confirm previous findings that PRRSV primarily replicates 
      in tonsils and is then shed into oral fluid. Therefore, oral fluid sampling may 
      be effective for the surveillance of PRRSV in breeding herds.
FAU - Trang, Nguyen Thi
AU  - Trang NT
AD  - Department of Veterinary Pathology, Faculty of Agriculture, University of 
      Miyazaki, Miyazaki 889-2192, Japan.
FAU - Hirai, Takuya
AU  - Hirai T
FAU - Yamamoto, Tsukasa
AU  - Yamamoto T
FAU - Matsuda, Mari
AU  - Matsuda M
FAU - Okumura, Naoko
AU  - Okumura N
FAU - Giang, Nguyen Thi Huong
AU  - Giang NT
FAU - Lan, Nguyen Thi
AU  - Lan NT
FAU - Yamaguchi, Ryoji
AU  - Yamaguchi R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140402
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
SB  - IM
MH  - Animals
MH  - Female
MH  - In Situ Hybridization/veterinary
MH  - Lung/virology
MH  - Male
MH  - Palatine Tonsil/virology
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Saliva/*virology
MH  - Salivary Glands/virology
MH  - Swine/virology
MH  - Virus Replication/physiology
PMC - PMC4178137
OTO - NOTNLM
OT  - acclimatization
OT  - localization
OT  - oral fluid
OT  - porcine reproductive and respiratory syndrome virus
OT  - tonsil
COIS- There is no conflict of interest.
EDAT- 2014/04/03 06:00
MHDA- 2016/01/16 06:00
PMCR- 2014/09/01
CRDT- 2014/04/03 06:00
PHST- 2014/03/03 00:00 [received]
PHST- 2014/04/01 00:00 [accepted]
PHST- 2014/04/03 06:00 [entrez]
PHST- 2014/04/03 06:00 [pubmed]
PHST- 2016/01/16 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - jvs.2014.022 [pii]
AID - 10.4142/jvs.2014.15.3.361 [doi]
PST - ppublish
SO  - J Vet Sci. 2014;15(3):361-7. doi: 10.4142/jvs.2014.15.3.361. Epub 2014 Apr 2.