PMID- 24692174
OWN - NLM
STAT- MEDLINE
DCOM- 20150526
LR  - 20140714
IS  - 1755-3245 (Electronic)
IS  - 0008-6363 (Linking)
VI  - 103
IP  - 2
DP  - 2014 Jul 15
TI  - Stretch-induced Ca2+ signalling in vascular smooth muscle cells depends on Ca2+ 
      store segregation.
PG  - 313-23
LID - 10.1093/cvr/cvu069 [doi]
AB  - AIM: Calcium is a key second messenger that can be mobilized from both the 
      extracellular medium and intracellular calcium stores. Pulmonary arterial smooth 
      muscle cells (PASMCs) respond to stretch by a calcium increase, a mechanism 
      enhanced during pulmonary hypertension (PH). We investigated the role of the 
      spatial organization between plasma membrane stretch-activated channels (SACs) 
      and intracellular calcium stores [sarcoplasmic reticulum (SR), mitochondria, and 
      lysosomes) in response to stretch. METHODS AND RESULTS: Studies were performed in 
      freshly isolated PASMCs from both control and two different rat models of PH 
      (chronically hypoxic and monocrotaline-treated rats). Co-immunolabellings 
      revealed that the subcellular segregation between each subtype of SR ryanodine 
      receptors (RyR1, RyR2, and RyR3), SERCA2 pumps (SERCA2a and SERCA2b), 
      mitochondria, or lysosomes in freshly isolated PASMCs differs from control and PH 
      PASMCs. In control PASMCs, stretching the membrane activates a Ca(2+) influx 
      through SACs. This influx is amplified by cell hyperpolarization, a calcium 
      release by subplasmalemmal RyR1 and is then buffered by mitochondria. In two 
      different PH rat models, the calcium response to stretch is enhanced due to 
      hyper-reactivity of SACs and a greater calcium amplification by all RyR subtypes. 
      CONCLUSION: The spatial organization of RyR and calcium stores in PASMCs is 
      important for cell signalling and plays a causal role in PH.
CI  - Published on behalf of the European Society of Cardiology. All rights reserved. (c) 
      The Author 2014. For permissions please email: journals.permissions@oup.com.
FAU - Gilbert, Guillaume
AU  - Gilbert G
AD  - Centre de Recherche Cardio-Thoracique de Bordeaux, Universite de Bordeaux, 
      Bordeaux F-33000, France Centre de Recherche Cardio-Thoracique de Bordeaux, 
      INSERM, U 1045, Bordeaux F-33000, France.
FAU - Ducret, Thomas
AU  - Ducret T
AD  - Centre de Recherche Cardio-Thoracique de Bordeaux, Universite de Bordeaux, 
      Bordeaux F-33000, France Centre de Recherche Cardio-Thoracique de Bordeaux, 
      INSERM, U 1045, Bordeaux F-33000, France.
FAU - Marthan, Roger
AU  - Marthan R
AD  - Centre de Recherche Cardio-Thoracique de Bordeaux, Universite de Bordeaux, 
      Bordeaux F-33000, France Centre de Recherche Cardio-Thoracique de Bordeaux, 
      INSERM, U 1045, Bordeaux F-33000, France CHU Bordeaux, Exploration Fonctionnelle 
      Respiratoire, Bordeaux F-33000, France.
FAU - Savineau, Jean-Pierre
AU  - Savineau JP
AD  - Centre de Recherche Cardio-Thoracique de Bordeaux, Universite de Bordeaux, 
      Bordeaux F-33000, France Centre de Recherche Cardio-Thoracique de Bordeaux, 
      INSERM, U 1045, Bordeaux F-33000, France.
FAU - Quignard, Jean-Francois
AU  - Quignard JF
AD  - Centre de Recherche Cardio-Thoracique de Bordeaux, Universite de Bordeaux, 
      Bordeaux F-33000, France Centre de Recherche Cardio-Thoracique de Bordeaux, 
      INSERM, U 1045, Bordeaux F-33000, France Centre de Recherche Cardio-Thoracique de 
      Bordeaux, 146 rue Leo Saignat, Bat TP - 2eme etage, 33076 Bordeaux Cedex, France 
      jean-francois.quignard@u-bordeaux.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140401
PL  - England
TA  - Cardiovasc Res
JT  - Cardiovascular research
JID - 0077427
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Ryanodine Receptor Calcium Release Channel)
RN  - 15662-33-6 (Ryanodine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Signaling/drug effects/*physiology
MH  - Hypertension, Pulmonary/metabolism
MH  - Male
MH  - Muscle, Smooth, Vascular/drug effects/*metabolism
MH  - Myocytes, Smooth Muscle/drug effects/*metabolism
MH  - Pulmonary Artery/metabolism
MH  - Pulmonary Stretch Receptors/metabolism
MH  - Rats, Wistar
MH  - Ryanodine/metabolism
MH  - Ryanodine Receptor Calcium Release Channel/metabolism
MH  - Sarcoplasmic Reticulum
OTO - NOTNLM
OT  - Calcium signalling
OT  - Osmotic shock
OT  - Pulmonary hypertension
OT  - Ryanodine receptor
OT  - Stretch-activated channel
EDAT- 2014/04/03 06:00
MHDA- 2015/05/27 06:00
CRDT- 2014/04/03 06:00
PHST- 2014/04/03 06:00 [entrez]
PHST- 2014/04/03 06:00 [pubmed]
PHST- 2015/05/27 06:00 [medline]
AID - cvu069 [pii]
AID - 10.1093/cvr/cvu069 [doi]
PST - ppublish
SO  - Cardiovasc Res. 2014 Jul 15;103(2):313-23. doi: 10.1093/cvr/cvu069. Epub 2014 Apr 
      1.