PMID- 24692327
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20141114
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 42
IP  - 12
DP  - 2014 Dec
TI  - Fluorescence in situ hybridization as an adjunct tool in the diagnosis of primary 
      and metastatic renal cell carcinoma in fine needle aspiration specimens.
PG  - 1013-23
LID - 10.1002/dc.23154 [doi]
AB  - We investigated the role of fluorescence in situ hybridization (FISH) in the 
      diagnosis of primary renal neoplasms and lesions suspicious for metastatic renal 
      cell carcinoma. Consecutive fine-needle aspiration biopsies (FNAB) of 39 renal 
      masses and 41 metastatic tumours suspicious for renal cell origin were assessed 
      with an immunohistochemical panel for CK7, RCC antigen, CD10, AMACR, PAX8, 
      vimentin, and CD117. In addition, FISH was performed using probes for chromosomes 
      1p, 3p, 7, 17, X, and Y. A total of 31 of 39 primary renal masses and 33 of 41 
      metastatic tumors suspicious for renal origin demonstrated typical cytological 
      and immunohistochemical (IHC) features of subtypes of renal neoplasms (40 clear 
      cell renal cell carcinoma (RCC), 20 papillary RCC, and 4 renal oncocytomas). FISH 
      analysis of 15 randomly selected cases each of primary and metastatic lesions 
      revealed chromosomal abnormalities consistent with the diagnosis in 73% of these 
      cases. Of 8 primary renal masses demonstrating atypical microscopic features and 
      noncontributory IHC profiles, FISH was helpful in subtyping 5 (62%) of these 
      lesions (2 clear cell RCC, 1 solid variant of oncocytic papillary RCC, 1 mixed 
      clear cell and papillary RCC, and 1 chromophobe RCC with papillary architecture). 
      Of 8 metastatic tumors clinically suspicious for renal cell origin and 
      supportive, but nondiagnostic IHC, FISH revealed supportive chromosomal changes 
      in 6 (75%) cases. In conclusion FISH analysis on FNAB material, even with limited 
      tissue, may be contributory to the diagnosis and subtyping of RCC in 
      diagnostically challenging biopsies.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Kos, Zuzana
AU  - Kos Z
AD  - Pathology and Laboratory Medicine, The Ottawa Hospital and University of Ottawa, 
      Ottawa, Ontario, Canada.
FAU - Williams, Phillip A
AU  - Williams PA
FAU - Belanger, Eric C
AU  - Belanger EC
FAU - Mai, Kien T
AU  - Mai KT
LA  - eng
PT  - Journal Article
DEP - 20140401
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - Oncocytoma, renal
SB  - IM
MH  - Adenoma, Oxyphilic/diagnosis/*pathology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Biopsy, Fine-Needle
MH  - Carcinoma, Renal Cell/diagnosis/*pathology/secondary
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods
MH  - Kidney Neoplasms/diagnosis/*pathology
MH  - Male
MH  - Middle Aged
OTO - NOTNLM
OT  - biopsy
OT  - fluorescence in situ hybridization
OT  - renal carcinoma
EDAT- 2014/04/03 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/04/03 06:00
PHST- 2013/11/01 00:00 [received]
PHST- 2014/03/13 00:00 [accepted]
PHST- 2014/04/03 06:00 [entrez]
PHST- 2014/04/03 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.1002/dc.23154 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2014 Dec;42(12):1013-23. doi: 10.1002/dc.23154. Epub 2014 Apr 
      1.