PMID- 24692493
OWN - NLM
STAT- MEDLINE
DCOM- 20141114
LR  - 20140402
IS  - 1559-6095 (Electronic)
IS  - 1559-6095 (Linking)
VI  - 2014
IP  - 4
DP  - 2014 Apr 1
TI  - High-throughput RNAi screening for germline apoptosis genes in Caenorhabditis 
      elegans.
PG  - 428-34
LID - 10.1101/pdb.prot080234 [doi]
AB  - Among the greatest tools that Caenorhabditis elegans can provide researchers are 
      the capabilities to perform high-throughput, genome-wide screens. Using bacterial 
      RNAi libraries, which cover the majority (>85%) of the worm genome, genes can be 
      rapidly and systematically evaluated for apoptosis phenotypes in the germline. 
      Screens can be designed to directly assess the levels of apoptotic corpses under 
      normal physiological conditions using transgenic strains expressing fluorescent 
      reporters that mark apoptotic bodies. Vital dyes that are selectively retained in 
      apoptotic cells, such as acridine orange (AO), can also be used to screen for 
      genes that regulate germline apoptosis. Using these reagents, screens can be 
      performed in wild-type worms or mutant backgrounds that suppress or enhance 
      apoptosis phenotypes. This protocol describes methods for designing and carrying 
      out high-throughput or targeted RNAi screens for germline apoptosis regulators.
FAU - Lant, Benjamin
AU  - Lant B
AD  - Developmental and Stem Cell Biology Program, The Hospital for Sick Children, 
      Toronto, Ontario M5G 1L7, Canada;
FAU - Derry, W Brent
AU  - Derry WB
LA  - eng
GR  - MOP79495/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140401
PL  - United States
TA  - Cold Spring Harb Protoc
JT  - Cold Spring Harbor protocols
JID - 101524530
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Caenorhabditis elegans/*genetics
MH  - Fluorescent Dyes/analysis
MH  - *Gene Expression Regulation
MH  - Gene Knockdown Techniques/*methods
MH  - Genetic Testing/*methods
MH  - Germ Cells/*physiology
MH  - RNA, Small Interfering/*genetics/metabolism
MH  - Staining and Labeling
EDAT- 2014/04/03 06:00
MHDA- 2014/11/15 06:00
CRDT- 2014/04/03 06:00
PHST- 2014/04/03 06:00 [entrez]
PHST- 2014/04/03 06:00 [pubmed]
PHST- 2014/11/15 06:00 [medline]
AID - 2014/4/pdb.prot080234 [pii]
AID - 10.1101/pdb.prot080234 [doi]
PST - epublish
SO  - Cold Spring Harb Protoc. 2014 Apr 1;2014(4):428-34. doi: 10.1101/pdb.prot080234.